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《Molecular Plant Breeding》 2018-10
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Tissue Culture and Cultivation Techniques of Pineapple Variety Ananas comosus

Wang Li;Huang Hongjian;Tan Peitao;Chen Xiaoling;Hu Yang;Lin Jiantao;Xinhui Forestry Science Institute;  
This paper solved the problem of s low breeding speed and easy to be affected by environment in Ananas comosus through rapid propagation of Ananas comosus and exploration of its cultivation techniques. With the tender stem tips and the base of Ananas comosus as explants, the optimum medium formula and culture method were studied in each stage of tissue culture of Ananas comosus combined with various experiment designs. The callus appeared when the disinfected explants of Ananas comosus had been cultured in the optimized MS medium for 20 days, and the callus could be induced to obtain complete plant. The suitable method for disinfection of the explants of Ananas comosus was as follows: sterilized for 1 min with 75% alcohol, washed by sterile water and treated by 0.1% mercuric chloride for 10 minutes. 6-BA 0.5 mg, L+NAA 0.5 mg/L+2,4-D 0.5 mg/L was the best medium for the induction of adventitious bud, and t he callus induction rate could be up to 77.1%. The suitable concentration of hormone for the development of adventitious bud was 6-BA: 0.5~1.5 mg/L, NAA: 0.5~1.5 mg/L.IBA 0.5 g/L +NAA 0.5 mg/L was the best substrate at the rooting stage, and the rooting rate was 94.4%. The survival rate of transferred seedling reached 98% in the proportion of grass peat: sand of 1∶2, which had the largest economic benefit and best effect. This method sol ved the problem of slow reproduction rate of Ananas comosus and could provide valuable technical data and reference for industrialized production of Ananas comosus.
【Fund】: 广东省江门市科技计划项目(20150030001088)资助
【CateGory Index】: S668.3
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