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《Molecular Plant Breeding》 2018-10
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Tissue Culture and Rapid Propagation of Cinnamomun burmannii

Ma Xiaoyu;Fu Minghui;Huang Silei;School of Chemical Engineering and Light Industry, Guangdong University of Technology;  
The study aimed to improve the cultivation efficiency of Cinnamomun burmannii trees, so that the seedling process is no longer limited by environmental factors such as seasons. The process of tissue and rapid propagation of Cinnamomun burmannii were explored in this study. Stem segment explants with axillary buds of Cinnamomun burmannii were inoculated in the medium of DCR with 6-BA(2 mg/L), NAA(0.05 mg/L) and sucrose(30 g/L) after sterilization and were cultivated one month under the conditions of(25±2)℃, 1 500 Lx illumination intensity and 16 h/d(6:00~22:00) to induce the formation of axillary bud. The result showed that the induction rate of buds was 89.83% and the average bud length was over 1.5 cm with lead stretched healthily. While when the induced axillary bud was inoculated in the medium of DCR with 6-BA(2 mg/L), NAA(0.05 mg/L) and sucrose(30 g/L) and cultivated for 40 d under the same conditions, the bud proliferation reached at 1.90 and all buds were significantly elongated which was more robust than before proliferation. When the robust shoots were inoculated in the rooting medium of DCR with IBA(1.5 mg/L) and sucrose(30 g/L), root could be observed after 21 d, and the rootage rate reached 80.95% after 30 d. As the roots extended and roots formed, buds gradually grew up to be aseptic seedlings and the height reached at 5 cm in 60 d. A new approach was adopted to regenerate the tissue culture of Cinnamomun burmannii in this study, and the bud induction rate, propagation coefficient and rooting rate of Cinnamomun burmannii were significantly increased, which might provide a theoretical foundation for the industrialized production of tissue culture and rapid propagation of Cinnamomun burmannii.
【Fund】: 广东省科技计划项目(2016A010105020);; 国家自然科学基金(21177029)共同资助
【CateGory Index】: S792.99
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