Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Molecular Plant Breeding》 2018-10
Add to Favorite Get Latest Update

Function Analysis of NDPK1 in Cell Division

Wang Rui;Li Shenghui;Sun Shiwei;Wang Fengru;Laboratory of Molecular Phytopathology, Department of Life Science, Agricultural University of Hebei;Department of Life Science, Hengshui University;  
To clear the function and the action mechanism of NDPK1 in cell division, we analyzed the plant height and stem diameter of the NDPK1 over-expressing transgenic Arabidopsis using over-expression techniques in this study. The size and morphology of the NDPK1 over-expressing transgenic Arabidopsis cells were observed by scanning microscope and the number of cells per unit area was counted. RT-PCR technology was used to test the expression level of the cell division related genes in the NDPK1 over-expressing transgenic Arabidopsis, and preliminarily analyze the regulation mechanism of NDPK1 in cell cycle. The results showed that the length of hypocotyl in NDPK1 over-expressing transgenic Arabidopsis was longer than Col-0 wild-type Arabidopsis thaliana.The number of hypocotyl cell in NDPK1 over-expressing transgenic Arabidopsis was larger than Col-0 wild-type Arabidopsis thaliana. The expression level of CycD2, CDC2 which could shorten cell division cycle in NDPK1 over-expressing transgenic Arabidopsis was higher than that in Col-0 wild-type Arabidopsis thaliana. In conclusion,NDPK1 could regulate the expression of CDC2 and CycD2 genes to promote the progress of cell division.
【Fund】: 河北农业大学大学生创新创业训练计划(201710086050);; 河北省自然科学基金(C2017204060)共同资助
【CateGory Index】: Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved