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《Molecular Plant Breeding》 2018-10
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Excavation and SSR Identification of Polyploid Germplasm Resources in Jujube Trees

Liu Yanwei;Li Meiyu;Zhu Jingle;Yang Liu;Gao Jiao;Kong Decang;Li Yingyue;Pang Xiaoming;National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University;China Paulownia Research Center;National Improved Cultivar Station of Jujube;  
In order to find the polyploidy germplasm, and to provide materials for polyploidy breeding of jujube, the study used flow cytometry(FCM) to detect the DNA contents in 580 germplasm resources of jujube in the National Improved Cultivar Station of Jujube in Cangzhou, Hebei, and further analyzed the SSR in identified polyploidy.The results showed that five accessions were revealed as triploid germplasm resources by FCM including‘Zanhuangdazao'and‘Jinglinyihaozao',andthreenewly-foundpolyploidy,named‘Shanxitedage',‘Hengshuibianzhi',and‘Zhenhuluzao'. All the rest of the germplasm resources were diploid, which indicated low diversity level of chromosome in jujube species. Later, 24 pairs of SSR primers were applied to analyze the identified triploid germplasm. The results showed that three different alleles were detected in 3~9 SSR loci in different germplasms,further supporting the identification results of flow cytometry. Flow cytometry was used in large-scale identification experiments of Chinese jujube ploidy in this study, which would provide a fast and efficient method for chromosome ploidy identification in jujube combined with SSR marker.
【Fund】: 科技部“十二五”国家科技支撑计划(2013BAD14B0302);; 科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(PXM2017_014207_000043)共同资助
【CateGory Index】: S665.1
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