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《Molecular Plant Breeding》 2018-10
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High-Throughput Sequencing Analysis of the Fungal Diversity in the Soil under the Fairy Ring Fruitbodies of Leucocalocybe mongolicum

Yang Yue;Wei Jie;College of Forestry, Inner Mongolia Agricultural University;  
Leucocalocybe mongolicum is a fungus full of high edible and medicinal value, but its natural production is very small. In addition, the domestication and cultivation technology of it has had no breakthrough up to now, leading to its danger of extinction. The aim of this study was to investigate the fungal community structure in the soils under the fairy ring fruitbodies during the fruiting period of L. mongolicum on Hulunbuir grassland, so as to provide reference for the domestication and cultivation of L. mongolicum. The soil samples under L. mongolicum were collected, and the DNA was extracted. The ITS2 region of r DNA was sequenced as the target research area. Illumina Hi Seq high-throughput sequencing and phylogeny were used to analyze the species diversity and richness in the soil under L. mongolicum. 86 866 clean data were obtained and 255 OTUs were identified. Dominant fungi in the soils under L. mongolicum were as follows according to the richness of OTU:Fusarium, Trichoderma, Cladosporium, Phoma, Leucocalocybe, Alternaria, Ascochyta, Lecythophora, Myrothecium and Preussia. A majority o f dominant genera in the soils under the fairy ring fruitbodies of L. mongolicum were cosmopolitan genus, and they belonged to saprophytism or parasitism, or available to both of these two nutrition patterns. These fungi could decompose the organic matter in soils or plants to provide necessary nutrients for L.mongolicum when it formed fruitbodies. The ecological relationship between L. mongolicum and these fungi as well as its trophic style need to be further studied using different methods.
【Fund】: 高等学校博士学科点专项科研基金(20131515120015)资助
【CateGory Index】: S154.3;S646
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