Effects of Different Exogenous Hormones on Rooting Process of Camellia oleifera Cuttings Based on Orthogonal Experiment
Chen Jiali;Yun Yong;Yang Lirong;Wang Chunmei;Yan Wuping;Chen Xuan;Weng Chunyu;Zheng Daojun;The Key Laboratory of Tropic Special Economic Plant Innovation and Utilization;Institute of Tropical Horticulture Research in Hainan Academy of Agricultural Sciences;Institute of Tropical Agriculture and Forestry, Hainan University;
In order to clarify the effect of different exogenous hormones on rooting process of Camellia oleifera cuttings and obtain the formula of exogenous hormones, orthogonal experiment was used to study the effects of different exogenous hormones on the rooting of C. oleifera cuttings. The survival rate, rooting rate of C. oleifera cuttings under different ratio of exogenous hormones were studied. The results showed that the rooting of C. oleifera was a mixed rooting type, and the highest mixed rooting rate in this study was up to 93.33% for the first time. The rooting morphogenetic indexes under different exogenous hormone treatments were higher than that of water control.The rooting rate, mixed rooting rate, callus area and the number of primary adventitious roots was significant or extremely significant difference, but there was no significant difference in survival rate and callus formation rate between different exogenous hormone treatments. The best treatment was A10(1 000 mg/L naphthyleneacetic acid+3 000 mg/L indolebutyric acid +0.25 mg/L 2,4-Dichlorophenoxyacetic acid +1 mg/L sodium nitrophenolate), the survival rate, rooting rate, the mixed rooting rate and the number of primary adventitious roots of cuttings was the highest, 100%, 90%, 86.67% and 11.09 respectively. The range analysis showed that the effect of four exogenous hormones on rooting mor phogenesis of C. oleifera cuttings were significantly different. The effect of naphthaleneacetic acid and sodium nitrophenolate on callus area, rooting rate and mixed rooting rate were greater than that of Indolebutyric acid. The effect of indolebutyric acid and sodium nitrophenolate on the number of primary adventitious roots were similar. Naphthylacetic acid had the greatest effect on callus production, followed by indolebutyric acid. It was suggested that the effect of mixed use of different exogenous hormones was better than that of single hormone on rooting of plant cuttings, and sodium nitrophenolate should be pay attention to cuttings, the research provides technical support for the widespread application of Camellia oleifera technology in production.
【Fund】： 2016年海南省重点研发计划专项(ZDYF2016066);; 2016年省属科研院所技术开发专项(KYYS-2016-07)共同资助
【CateGory Index】： S794.4
【CateGory Index】： S794.4