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《Journal of Clinical Hepatology》 2000-01
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HDAG AND HBSAG/HBCAG EXPRESSION IN LIVER TISSUES FROM PATIENTS WITH HEPATITIS D BY IMMUNOHISTOCHEMICAL TECHNIQUE

Gu Xiaohong, Li Qifen Department of gastroenterology Daping Hospital, The Third Military Medical University, Chongqing 400042  
Objective To explore the relationship between expression of hepatitis D virus antigen(HDAg)and HBsAg/HBcAg in liver tissues of the patients with hepatitis D(HD). Methods HDAg and HBsAg/HBcAg in paraffin-embedded liver tissues from 79 liver biopsies of autopsy samples from the patients with hepatitis D virus (HDV) and hepatitis B virus(HBV)superinfection and from 52 patients with hepatitis B virus infection alone were studied by immunihistochemical double labelling techniques. Results The detection rates for HBsAg and HBcAg HD in patients were 81% and 71%,lower than that of hepatitis B( 94% ,92%) (P0.05 or 0.01) . HDAg was mainly expressed in nuclei and cytoplasm of hepatocyte,HDAg positive cells were obviously aggregated in periportal region,especially at the edges of piecemeal necrosis, moreover, strong expression of HDAg was shown in the bollooning degeneration hepa-tocytes and hepatocytes near piecemeal necrosis region. HBsAg was mainly expressed in cytoplasm of hepatocytes,and positive cells distribution were similar to that of HDAg positive cells. HBcAg was mainly expressed in unclei of hepatocytes, positive cells distribution were alone of focal type(3 - 5 cells). Double labelling studies showed that HDAg and HBsAg were mostly expressed in the same hepatocytes,and positive cells of HDAg and HBsAg were mostly found to locate in the same area of liver tissus,but HDAg seldom coexisted with HBcAg, respectively the expression degrees and positive cells distribution of HDAg and HBsAg were all obviously associated with the grades of pathological damage of liver tissues and necroinflammatory activity (P 0.01) . Conclusion HDV infection can inhibit replication of HBV genome or expression of HBcAg. Besides of interactions beteween HBV and HDV, it is possible that direct cyto-toxicity of HDV may play an impotant pathogenetic role in pathogenetic mechanisms of HDV.
【CateGory Index】: R512.6
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