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《Journal of Clinical Hepatology》 2000-01
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HEPATITIS B VIRUS SURFACE AND CORE GENE-BASED VACCINE IMMUNIZATION IN BALB/C MICE

Wang Quan-Chu , Zhou Yong-Xing, Yao Zhi-Qiang ,et al. Department of Infectious Disease , Tangdu Hospital, the Fourth Military Medical University, Xi' an 710038  
Objective To study HBV surface and core gene-based vaccine immunization in mice. Methods The HBV surface gene and core gene were cloned into an enkaryotic expression vector(pCR3. 1), respectively. The Balb/c mice were immunized intramuscularly with pCR3.1-S and pCR3.1-C. Each mouse was boosted 2 weeks after immunization. The anti-HBs and anti-HBc antibody were detected by ELISA.and the stimulatory index of splenocytes of mice immunized to HBsAg of HBcAg was measured by MTT method. Results The titers of special antibody and the stimulatory index of splenocytes from mice immunized with PCR3.1-S and pCR3.1-C were significantly higher than that of control. SI of immunized mice injected with pCR3.1-C was higher than that of pCR3.1-S group. Conclusion This study demonstrates that HBV C gene and S gene may elicit humoral and cellular immune response in mice.
【Fund】: 国家自然科学基金(NO.39770065)
【CateGory Index】: R392
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