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《Journal of Clinical Hepatology》 2003-01
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Detection of transfusion transmitted virus(TTV) infection and its partial gene cloning and sequencing in Shandong

Yu Jian- guo, Shang Qinghua, Ren Hao, et al. Liver Research Center, Department of infectious Diseases, Chinese PLA 88 th Hospital, Taian 271000, China.  
Objective To investigate the prevalence of TTV infection and genetic variation of TTV isolated in Shandong Province. METHODS TTV DNA were amplified and detected by PCR from sera of patients with NonA - G hepatitis and hepatocellular carcinoma. The PCR products was cloned and sequenced. RESULTS TTV DNA was positive in 11 of 26 patients(42.3%)with nonA - G hepatitis. Partial gene of two TTV isolates(TTVSD4, TTVSD5) was cloned, sequenced and compared with known sequence of TTV isolates in Japan (ABOO8394) reported by Okamoto.The nucleotide homology were 99% and 100% .TTV DNA was positive in 3 of 12 patients(25%) with hepatocellular careinoma. Partial gene of TTV isolates (TTVSD6) was cloned, sequenced and compared with known sequence of TTV isolated in Japan( ABOO8394). The nucleotide homology was 99 % . CONCLUSION The TTV infection rate is high in Shandong. TTV might be hepatotropic, maybe correlated with the impariment of liver function. So, it is likely to be the pathogen of nmA - G hepatitis.
【CateGory Index】: R346
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