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《Journal of Clinical Hepatology》 2003-03
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Effect of HCV envelope gene on core DNA vaccine in mice

Feng Zhilhua, Zhou Yongxing , Du Dewei, et al. Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xian 710038, China  
Objective To investigate the enhancing effect of envelope gene(E1E2)on immune responses of HCV core(C) gene induction in mice. Methods Balb/c mice were immunized with the recombinant plasmid containing HCV core(pHCV-C) or full-length structural gene (pHCV-CE1E2).HCV C specific antibody in the sera was measured with FLISA. CTL activity was measured by standard 4h~(51) Cr release assays. Spleen cells from experimental and control mice were extracted with routine method. The mouse myloma SP2/0 cells expressing HCV C antigen serve as target cells. Result The plasmid pHCV-CE1E2 didn't enhance HCV core-specific antibody level and CTL activity compared with control mice immunized with pHCV-C. Conclusion The envelope gene could not improve HCV core specific antibody level and CTL activity. However, we cannot exclude from these data that there are not CTL epitopes in HCV envelope gene region.
【CateGory Index】: R392
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Chinese Journal Full-text Database 4 Hits
1 FENG Zhi-Hua, ZHOU Yong-Xing, LI Jin-Ge, WANG Quan-Chu, DU De-Wei, JIA Zhan-Sheng, JIAO Chen-Song Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China;Cytokine enhances immune responses to DNA vaccine of HCV[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-07
2 WEI San-Hua, YIN Wen, LEI Ying-Feng, HU Xing-Bin, YANG Jing, L Xin, SUN Meng-Ning, XU Zhi-KaiDepartment of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Establishment of stably transfected P815 cell line expressing multi-epitope gene of hepatitis C virus[J];Journal of the Fourth Military Medical University;2006-01
3 WANG Shui-lian1,YANG Li-guo2,XUE Li-qun1,CAO Ying-chun1,CHEN Xiao-jun1(1.College of Veterinarian,HNAU,Changsha 410128,China;2.College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China);Effect of Different Factors on Immune Response of Inhibin Gene in Beef Cattle[J];Journal of Hunan Agricultural University(Natural Sciences);2006-06
4 Zhang Dekun 1, Yang Liguo 1, Zhang Honglin 1, Wang Wen 2, Wunarhan 2, Ji Ping 3, Gu Wuchang 3 (1. Nanjing Agricultural University Animal Breeding and Reproduction Laboratory, Nanjing 210095,China; 2. Xinjiang Supervision of Animal Epidemic Prevention and Control Center, Wulumuqi 830017, China; 3. Akesu Supervision of Animal Epidemic Prevention and Control Center, Akesu 843000, China);Inhibin gene immunization to induce sheep twinning[J];Journal of China Agricultural University;2004-04
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