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《Journal of Clinical Hepatology》 2003-04
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Study of transcription and deavage in vitro of HDV with HBV-speciffc hammerhead ribozyme

Dai Wei, Zhou Rong, Dong Yanrong ,et al. Donghu Hospital, Shenzhen Guangdong ,518020  
Objective To study the effect of hepatitis B virus (HBV) cleavage by HBV specific ribozyme (RZ) and recombi-nant hepatitis D virus (rHDV) harbouring hammerhead RZ(rHDVRZA,rHDVRZB and rHDVRZC) .Methods The pTA-HBV vector including 831bp HBV C gene fragment was digested with BamHI and cloned under the control of T7 promoter, and 32P labeled HBV C transcript was incubated with gel-purified rRZ,rHDVRZA,rHDVRZB and rHDVRZC at different temperature and autoradiographed after denaturing gel-electrophoresis.Results Our results showed that rRZ,rHDVRZA,rHDVRZB and RHDVRZC were active at 37℃. Conclusion rHDV could serve as a vector for the delivery of the RZ specific for HBV cleavage. Our data demonstrated the value of rRZ as potential therapeutic agents for HBV infection.
【Fund】: 本课题受广东省自然科学基金资助。
【CateGory Index】: R346
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