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《Journal of Clinical Hepatology》 2008-01
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The effect of non-structural protein 4B of hepatitis C virus on LO2 cell proliferation and expression of cyclinD1 in vitro

YANG Chun,LI Changping,CHEN Feng,et al.Department of Gastroenterology,Affiliated Hospital of Luzhou Medical College,Luzhou 646000,China  
Objective To investigate the effect of non-structural protein 4B of hepatitis C virus on LO2 cell proliferation and expression of cyclinD1 in vitro.Methods The plasmid of PCXN2-NS4B and the empty vector PCXN2 were transfected into LO2 liver cells with liposome.The transfection was confirmed by reverse transcription PCR.The cell growth was detected by MTT.Flow cytometry was used to determine the cell cycles and the cyclinD1 expression was determined by immunohistochemistry.Results Stable expression of the recombinant plasmid was found in transfected LO2 liver cells;HCV NS4B promoted proliferation of LO2 cells;Flow cytometry analysis showed that 65% of L02 cells was in G0/G1,27% in S and 7% in G2/M phase in PCXN2 transfection,while 46% cells in G0/G1,36% in S and 16 in G2/M phase in cells with PCXN2-NS4B transfection.The expression of cyclinD1 was 14% in PCXN2-NS4B and 9% in PCXN2 transfection(P0.01).Conclusion HCV NS4B promotes the expression of cyclinD1 expression,which might improve the proliferation of hepatic cells.
【Fund】: 四川省教育厅资助项目(20003531)
【CateGory Index】: R512.6
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