Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Hepatology》 2008-01
Add to Favorite Get Latest Update

The effect of non-structural protein 4B of hepatitis C virus on LO2 cell proliferation and expression of cyclinD1 in vitro

YANG Chun,LI Changping,CHEN Feng,et al.Department of Gastroenterology,Affiliated Hospital of Luzhou Medical College,Luzhou 646000,China  
Objective To investigate the effect of non-structural protein 4B of hepatitis C virus on LO2 cell proliferation and expression of cyclinD1 in vitro.Methods The plasmid of PCXN2-NS4B and the empty vector PCXN2 were transfected into LO2 liver cells with liposome.The transfection was confirmed by reverse transcription PCR.The cell growth was detected by MTT.Flow cytometry was used to determine the cell cycles and the cyclinD1 expression was determined by immunohistochemistry.Results Stable expression of the recombinant plasmid was found in transfected LO2 liver cells;HCV NS4B promoted proliferation of LO2 cells;Flow cytometry analysis showed that 65% of L02 cells was in G0/G1,27% in S and 7% in G2/M phase in PCXN2 transfection,while 46% cells in G0/G1,36% in S and 16 in G2/M phase in cells with PCXN2-NS4B transfection.The expression of cyclinD1 was 14% in PCXN2-NS4B and 9% in PCXN2 transfection(P0.01).Conclusion HCV NS4B promotes the expression of cyclinD1 expression,which might improve the proliferation of hepatic cells.
【Fund】: 四川省教育厅资助项目(20003531)
【CateGory Index】: R512.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 2 Hits
1 CHEN Xia~ 1 ,LI Chang-ping~ 1 ,XU Jian-yu~ 2 ,CHEN Feng~ 3 1.Department of Digestive disease,Affiliated Hospital of Luzhou Medical College,Luzhou 646000,China; 2.Department of Digestive Disease,The Suining People's Hospital; 3.Department of Infectious disease, Affiliated Hospital of Luzhou Medical College;The Effect of HCV NS4B on Expressions of c-myc Protein and ras Protein in Hepatic Cells[J];Cancer Research on Prevention and Treatment;2006-10
2 LIU Yan, CHENG Jun, WANG Jianjun, et al. Gene Therapy Research Center, Institute of Infectious Diseases, The 302 Hospital of PLA, Beijing 100039, China;Cloning of genes transactivated by nonstructural protein 4B of hepatitis C virus[J];Chinese Hepatology;2005-01
【Co-citations】
Chinese Journal Full-text Database 1 Hits
1 YANG Chun,LI Chang-ping,CHEN Feng,et al.Department of Digestive,Affiliated Hospital of Luzhou Medical Colleage,Luzhou,Sichuan 646000,China;The effect of hepatitis C Virus non-structural protein 4B on cell cycle and expression of PCNA in hepatic cells[J];Sichuan Medical Journal;2008-01
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Du Chengyou,Yang Zukei,Wang Honglin,et al.Department of Hepatobiuiary Surgery.First Affiliated Hospital.Chongqing University of Medical Sciences,Chongqing,400016.;LIVER CANCER IN THE YOUNG ADULT——A REPORT OF 24 CASES[J];JOURNAL OF HEPATOBILIARY SURGERY;2000-03
2 Zhang Zhijian,Yang Jiamei,Wu Mengchao.Eastem Hepatobiliary Surgery Hospital,Second Military University,Shanghai 200433;DISCUSSION OF ESTABLISHING THE CRITERLA OF CURATIVE HEPATECTOMY FOR HEPATOCELLULAR CARCINOMA[J];JOURNAL OF HEPATOBILIARY SURGERY;1999-03
3 XU Qing *,ZHANG Yi-xin,WANG Zhi-wei,et al. * Department of General Surgery,the Affiliated Hospital of Nantong University,Nantong 226000;Clinical significance of microvessel count in hepatocellular carcinoma[J];Journal of Hepatopancreatobiliary Surgery;2005-01
4 Jiang Jingui,Zhou Xiaojun,Sun Guiqing et al Department of Pathology,Jinling H ospital(Nanjing,2 1 0 0 0 2 );Value of CD34 immunoreactivity in the diagnosis of hepatocellular carcinoma[J];BULLETIN OF JINLING HOSPITAL;1997-04
5 REN Yong-Ya (Department of Pathology,Nanjing Medical University,Nanjing 210029,China);Core protein of hepatitis C virus activates expression of iNOS and enhances DNA damage[J];Bulletin of the Academy of Military Medical Sciences;2006-06
6 CHEN Jian-guo.Department of Epidemiology,Qidong Liver Cancer Institute,Qidong 226200,China;Advances in the Etiology and Prevention for Primary Liver Cancer[J];Journal of Qilu Oncology;2003-11
7 Zhu Lixin, Geng Xiaoping, Fan ST. Department of Surgery, Affiliated Hospital of Anhui Medical University, Hefei 230022;Comparing the expression of CD34,CD31,von willebrand factor,type Ⅳ collagen and laminin in hepatocellular carcinoma[J];Digestive Surgery;2003-03
8 SUN Huichuan, TANG Zhaoyou, MA Zengchen. Liver Cancer Institute & Zhong Shan Hospital, Shanghai Medical University, Shanghai 200032;Clinico pathological factors influencing the disease-free survival of patients with hepatocellular carcinoma after radical operation[J];CHINESE JOURNAL OF HEPATOBILIARY SURGERY;2000-01
9 WAN Yun-le DING Wei ZHENG Shu-sen. Department of Hepatobiliary Surgery,Second Hospital of Sun Yat-sen University,Guandzhou 510120,China;Significance of Survivin and Ki-67 expression in primary gallbladder carcinoma[J];Chinese Journal of Experimental Surgery;2006-08
10 ZHOU Jian, FAN Jia, TANG Zhao-you, WU Zhi-quan, ZHOU Xin-da, MA Zeng-chen, TAN Chang-jun, SHI Ying-hong, YU Yao, QIU Shuang-jian. Liver Cancer Institute, Zhongshan Hospital, Fudam University, Shanghai 200032, China;Time dependency of factors influencing survival of hepatocellular carcinoma patients with portal vein tumor thrombosis after surgery[J];National Medical Journal of China;2006-42
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 LUO Dan, LIU Qi Fu, SU Jian Jia, QIN Guo Zhong, DUAN Xiao Xian, QIN Liu Liang, LI Yuan, HUANG Ding Riu (Guangxi Cancer Institute, Nanning 530021);In situ expression of oncogenes during multiple chemical hepatocarcinogenesis in rats[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;1998-02
2 LIU Yan, CHENG Jun, LU Yin-ying, WANG Gang, MOUJing-shong, LI Li, ZHANG Ling-xia, CHEN Ju-mei. Gene Therapy Research Center, Institute of Infectious Diseases, 302nd Hospital of People's Liberation Army, Beijing 100039, China;Cloning of genes transactivated by hepatitis B virus X protein[J];Chinese Journal of Hepatology;2003-01
3 LIU Yan,CHENG Jun,SHAO De zhi,WANG Lin,ZHONG Yan wei,DONG Jing,LI Ke,LI Li.Gene Therapy Research Center, Institute of Infectious Diseases,The 302nd Hospital, Beijing 100039,China;Synergetic transactivating effect of HCV core and HBV X proteins on SV40 early promoter/enhancer[J];Chinese Journal of Experimental and Clinical Virology;2003-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved