Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Hepatology》 2008-01
Add to Favorite Get Latest Update

Effect of NS-398 on the proliferation and apoptosis of HepG2 cell line

WU Guangyang,LI Jiansheng,XU Geliang,et al.Department of General Surgery,Affiliated Provincial Hospital of AnHui Medical University,Hefei 230001,China  
Objective To investigate the effects of NS-398,an cyclooxygenase-2 selective inhibitor,on the proliferation and apoptosis of HepG2 cells.Methods The proliferation of cells was detected by MTT assay.The cell cycle and apoptosis associated with bcl-2 were detected by flow cytometry.Results NS-398 inhibited the proliferation of human HepG2 cells at a dose-dependent manner.When interfered with NS-398 at a dose of 20,40,80 and 160μmol/L for 48h,the inhibiting rates were 6.72%,16.21%,20.86% and 25.34%,respectively;and when interfered with 160μmol/L NS-398 for 24h,48h and 72h,the cells of G0/G1 from 76.07 ± 0.75% reduced to 62.27 ± 0.74%,59.17 ± 1.47% and 53.03 ± 1.60%,respectively(P 0.05);the cells of S stage from 11.40±0.79% increased to 13.23±0.81%,16.20±1.95% and 16.60±1.25%,respectively(P0.05).The cell of G2/M stage was not markedly changed;The apoptotic cells increased with the apoptosis rates of 8.47%,16.3% and 23.9%,respectively.The expression of bcl-2 decreased obviously as compared with control group(P0.01).Conclusion NS-398 can inhibit the proliferation and induce apoptosis of HepG2,which might be related to the down regulated expression of bcl-2.
【CateGory Index】: R735.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 GAO Jian,LI Jian-sheng,XU Ge-liang,et al.Department of hepatic surgery,Affiliated Provincial Hospital,Anhui Medical University,Hefei 230001,China;COMBINED INHIBITORY EFFECTS OF CELECOXIB AND HMG-COA REDUCTASE INHIBITOR ON THE GROWTH OF HUMAN HEPATOCELLAR CARCINOMA CELLS IN A XENOGRAFT MODEL[J];Journal of Hepatobiliary Surgery;2010-02
Chinese Journal Full-text Database 1 Hits
1 CHEN Jian-Guo1,SONG Xin-Ming2(1.Qidong Liver Cancer Institute,Qidong 226200,China;2.Institute of Population,Beijing University,Beijing 100871,China);An Evaluation on Incident Cases of Liver Cancer in China[J];Bulletin of Chinese Cancer;2005-01
Chinese Journal Full-text Database 10 Hits
1 HAO Ying,DONG Yonghe(Baotou Medical College,Baotou 014010,China);Analysis of the Characteristics of 294 In-patients with Liver Cancer in Baotou[J];Journal of Baotou Medical College;2008-01
2 WU Jing,LUO Rong-cheng,ZHANG Hua,CUI Yan-zhi Department of Oncology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Inhibitory effect of sorafenib combined with arsenic trioxide on hepatocellular carcinoma cells[J];Journal of Southern Medical University;2008-04
3 TAN Sheng-kui,QIU Xiao-qiang,YU Hong-ping,et al. Department of Preventive Medicine of Guilin Medical College,Guilin 541001,China;Logistic regression analysis on risk factors of primary hepatic carcinoma in Guangxi[J];Acta Medicinae Sinica;2008-01
4 Feng Liang,Zeng Xiaoyun,Tan Sheng kui,et al.(Public Health College,Guangxi Medical University, Nannin 530021 China);STUDY ON RISK RELATIONSHIP BETWEEN HEPATITIS B VIRUS INFECTION AND PRIMARY HEPATIC CARCINOMA[J];Journal of Guangxi Medical University;2007-06
5 ZHANG Chun-yan, HUANG Tian-ren, ZHANG Zhen-quan,YU Jia-hua,HE Zhen-fang,ZHOU De-nan,LI Ji-lin,YE Si-yuan,DENG Wei,SHENG Ying,LIANG An-min,TANG Bu-jian.Guangxi Tumour Institute(Nanning 530021),China;Study on HBV infection of the cancer prevention screening population in the primary liver cancer high incidence area of Guangxi[J];Guangxi Medical Journal;2006-12
6 Zhi-Xing Guo,1,2 Wei Wei,1,2 Chong Zhong,1,2 Ming Shi,1,2 Min-Shan Chen1,2 and Rong-Ping Guo1,21. State Key Laboratory of Oncology in South China,Guangzhou,Guangdong,510060,P. R. China 2. Department of Hepatobiliary Oncology,Sun Yat-sen University Cancer Center,Guangdong,Guangzhou,510060,P. R. China;Correlation of preoperative neutrophil-to-lymphocyte ratio to prognosis of young patients with hepatocellular carcinoma[J];Chinese Journal of Cancer;2009-11
7 ZHU Xiao-jing, ZHANG Jian-ping1*, LIU Yue-fang, XU Lin-feng1, ZHANG Hui-lin, LI Yu-hua, CHEN Xi-min, FENG Zhen-qing, GUAN Xiao-hong2 (Department of Pathology, NJMU, Nanjing 210029; 1Department of General Surgery, the Second Affiliated Hospital of NJMU, Nanjing 210011,2Institute of Medical Molecular Biology, NJMU, Nanjing 210029, China);Expression of annexin II in hepatocellular carcinoma[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2006-08
8 BAI Rui-xia,WANG Wen-li(Department of Biochemistry and Molecular Biology,Inner Mongolia Medical College,Hohhot 010059 China);APPLICATION OF PROTEOMICS IN THE STUDY OF HEPATOCELLULAR CARCINOMA[J];Acta Academiae Medicinae Neimongol;2006-S1
9 ;Problems and Survey on Medical Image Processing[J];Software Guide;2009-04
10 Liu Huan1 Tang Liling1,2 Sun Caixin2 Mi Yan2 Yao Chenguo2 Li Chengxiang2 Wang Yuanliang1 1(Bioengineering College of Chongqing University, Chongqing 400044,China) 2( Key Laboratory of High Voltage Engineering and Electrical New Technology Under the State Ministry of Education, Chongqing University,Chongqing 400044,China);The Study of Apoptosis and Mechanism of Cells Exposed to Steep Pulse[J];Journal of Biomedical Engineering;2008-03
China Proceedings of conference Full-text Database 1 Hits
1 ;Risk evaluation of hepatic distomiasis induce Primary Hepatocellular carcinoma。[A];[C];2008
Chinese Journal Full-text Database 1 Hits
1 Zheng-Dong Fang,Jian-Sheng Li, Department of General Surgery, the Affiliated Anhui Provincial Hosptial of Auhui Medical University, Hefei 230001, Anhui Province, China Xiu-Cai Xu, Department of Central Laboratory, the Affiliated Anhui Provincial Hosptial of Auhui Medical University, Hefei 230001, Anhui Province, China;Effects of selective cyclooxygenase-2 inhibitor on invasion of human hepatocelluar carcinoma cells[J];World Chinese Journal of Digestology;2006-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhu Jian, Yao Hongyu, Zhang Yonghui, et al. (Qidong Liver Cancer Institute , Qidong Jiangsu 226200, China);An Analysis of Incidence Trend for Esophagus Cancer During 1972~2000 in Qidong[J];Medical Journal of Communications;2002-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved