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《Journal of Clinical Hepatology》 2008-01
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Effect of NS-398 on the proliferation and apoptosis of HepG2 cell line

WU Guangyang,LI Jiansheng,XU Geliang,et al.Department of General Surgery,Affiliated Provincial Hospital of AnHui Medical University,Hefei 230001,China  
Objective To investigate the effects of NS-398,an cyclooxygenase-2 selective inhibitor,on the proliferation and apoptosis of HepG2 cells.Methods The proliferation of cells was detected by MTT assay.The cell cycle and apoptosis associated with bcl-2 were detected by flow cytometry.Results NS-398 inhibited the proliferation of human HepG2 cells at a dose-dependent manner.When interfered with NS-398 at a dose of 20,40,80 and 160μmol/L for 48h,the inhibiting rates were 6.72%,16.21%,20.86% and 25.34%,respectively;and when interfered with 160μmol/L NS-398 for 24h,48h and 72h,the cells of G0/G1 from 76.07 ± 0.75% reduced to 62.27 ± 0.74%,59.17 ± 1.47% and 53.03 ± 1.60%,respectively(P 0.05);the cells of S stage from 11.40±0.79% increased to 13.23±0.81%,16.20±1.95% and 16.60±1.25%,respectively(P0.05).The cell of G2/M stage was not markedly changed;The apoptotic cells increased with the apoptosis rates of 8.47%,16.3% and 23.9%,respectively.The expression of bcl-2 decreased obviously as compared with control group(P0.01).Conclusion NS-398 can inhibit the proliferation and induce apoptosis of HepG2,which might be related to the down regulated expression of bcl-2.
【CateGory Index】: R735.7
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