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《Journal of Clinical Hepatology》 2008-01
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The apoptosis and expression of caspase-3 of hepatocytes in alcohol and/or high fat diet-induced fatty liver disease in rats

LIU Huiling,LU Wei,MEI Mei,et al.Department of Gastroenterology,Haihe Hosipital,Tianjin 300350,China  
Objective To study the apoptosis and expression of caspase-3 of hepatocytes in alcohol and/or high fat diet-induced fatty liver disease in rats.Methods Forty-six male Wistar rats were randomly divided into control,alcohol,fat-rich diet and alcohol/fat-rich diet group and fatty liver disease model was made by feeding alcohol and/or fat-rich diet.The serum AST,ALT,CHO,TG and FFA were detected;the expression of caspase-3 mRNA and caspase-3 protein in hepatic tissues was assayed by RT-PCR and by immunohistochemistry,respectively;the apoptosis was detected by TdT-mediated x-dUTP nick end labeling and ultrastructural observation.Results The serum ALT and AST in experiment groups were increased(P0.05).The apoptosis index in alcohol,fat-rich diet and alcohol/fat-rich diet group were 2.16±0.43,2.33±0.60,3.93±0.80,much higher than that in control group(1.07±0.27,P0.05)/The expression of caspase-3 protein and mRNA was significantly increased io the liver tissues of rats with fatty liver disease,and the highest expression was found in alcohol /fat-rich group(P0.01).Factorial analysis indicated that alcohol and high fat diets had positive effect on expression of caspase-3 mRNA(P=0.00).Conclusion Alcohol and fat-rich diets can induce the apoptosis of hepatocyte,which might aggravate the injury of liver.
【CateGory Index】: R575.5
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【Co-references】
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