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《Guangdong Agricultural Sciences》 2017-06
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Extraction optimization of main ingredients from Berchemia polyphylla var. leioclada.

TANG Yuan-jiang;SHANG Yi-shun;ZHOU Si-xuan;YANG Yue-qian;ZHANG Tao;YU Bo;Guizhou Hongyu Animal Husbandry Technology Development Ltd;Guizhou Institute of Animal Husbandry and Veterinary Medicine;  
Berchemia polyphylla var. leioclada. is a national medicine plant. In order to find the optimum condition of rutin and quercetin from B. polyphylla var. leioclada. reflux extraction method and surface response analysis were used,ethanol concentration,extraction temperature,solid-liquid ratio factors and their ineraction and extracting time were designed to optimize the extracting process. The results showed that the best extraction condition of rutin and quercetin were selected with 73.72% alcohol,60.52℃ and 32.21 m L/g of solid-liquid ratio,extracting time was 3 h. The extraction rates of rutin and quercetin were 18.55 and 0.36 mg/g,respectively. Through experiment study,the average of rutin and quercetin were 18.48 and 0.35 mg/g,respectively,which had a lillte error to the theory value. The results provide a theoretical basis for further development and use of rutin and quercetin from B. polyphylla var. leioclada.
【Fund】: 贵州省科学技术重大专项(黔科合[2013]6014);; 贵州省农业科技计划建设项目(黔科合农G字[2015]4001)
【CateGory Index】: R284.2
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