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《Guangdong Journal of Animal and Veterinary Science》 2010-01
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Clone and variation analysis of ORF3 gene of Porcine Reproductive and Respiratory Syndrome virus strain TS isolated form Hebei district

Wang Jiao,Zhao Zekun,Wang Kun,Sun Jiguo(College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001,China)  
One PRRS virus strain TS was isolated from pigs of Hebei Tangshan after inoculated to Marc-145 cell.The full-length of ORF3 gene of PRRSV strain TS was amplified by RT-PCR with a pair of specific primers designed according to the ORF3 sequence of PRRSV JXA1 strain,and then cloned into plasmid pMD19-T.The recombinant plasmid named as pMD-GP3 was sequenced and analyzed.The sequence was compared with that of other PRRSV strains in GenBank by using DNAStar software and the phylogenetic tree was drawn.It laid the foundation for further study for genetic engineering vaccine and diagnostic reagent for PRRSV.
【Fund】: 河北省重大技术创新项目(07221008Z)
【CateGory Index】: S852.65
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