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《Guangdong Journal of Animal and Veterinary Science》 2014-01
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Comparation of Three Methods for Detection of Antibodies to H_1N_1 Swine Influenza Virus

Yang Qian;Xue Chunyi;Zhu Daozhong;Li Xiaoming;Cao Yongchang;State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University;Technical Center,Guangdong Entry-Exit Inspection and Quarantine Bureau;  
HA protein, virus-like liposomes, and virus-like particles, expressed by Bac-to-Bac baculovirus expression system, were coated as antigens to detect the HIN1 swine influenza virus antibody using indirect enzyme-linked immunosorbent assay(ELISA). The results of specificity experiments showed that the single-expressed HA protein and virus-like liposomes had good specificity, but the virus-like particles could not distinguish different subtypes of influenza virus. The results of sensitivity experiments showed that virus-like liposomes had the best sensitivity. The results of repetitive experiments showed that the repetitiveness of the three methods was high, and the coefficient of variation within-run and between-run were less than 1%. The results of stability experiments showed that three antigens could be stable at 4 ℃ for at least one year. In a word, the virus-like liposome has strongest immunoreactivity, and is safer than the inactivated whole influenza virus. Thus it represents a promising method in detection of H1N1 antibodies.
【Fund】: 国家质检总局科技计划项目(2011IK004)
【CateGory Index】: S858.28
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