Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《International Journal of Pediatrics》 2006-03
Add to Favorite Get Latest Update

Polymorphism of Human Cytomegalovirus UL142 Gene in Low Passage Clinical Isolates

LU Ying*,RUAN Qiang,HE Rong,et al.*Virus Laboratory,2nd Clinical Hospital,China Medical University,Shenyang 110004,China Corresponding author:RUAN Qiang,110004,Virus Laboratory,The 2nd Clinical Hospital,China Medical University,Shenyang,China  
Objective To investigate the polymorphism of human cytomegalovirus UL142 gene in low passage isolates and try to find the relationship between the polymorphism and different pathogenesis of congenital HCMV infection.Methods PCR was performed to amplify the entire HCMV UL142 gene region of 27 clinical isolates,which had been proven containing detectable HCMV-DNA by FQ-PCR.PCR amplification products of 13 isolates were sequenced directly and the sequence data was analysed.Results 13 among 27 isolates were amplified successfully and the positive rate was 48.2%.By comparison with Toledo sequence,the length of UL142 ORF in all 13 clinical isolates was similar to that of Toledo,921 bp in size,encoding 307amino acids' protein.All of the DNA sequence variations were nucleotide substitutions,neither insertions nor deletions were detected.Alignment comparison of the UL142 sequences with that of Toledo revealed that nucleotide and amino acid sequence homologies were 94.4%-95.9% and 89.9%-92.5% respectively.Three kinds of secondary structure had been found.Most functional motifs of UL142 protein were highly conserved although several strains had deleted or additional sites.The phylogenetic trees based on UL142 DNA and amino acid sequence of HCMV demonstrated that 13 clinical isolates could be divided into 3 gene groups.Conclusion All DNA and deduced amino acid sequences of UL142 gene shared great similarity among HCMV clinical strains regardless of their polymorphism.No linkage was found between diversities of UL142 gene and the outcomes of congenital HCMV infection.
【Fund】: 国家自然科学基金资助项目(30170986)
【CateGory Index】: R373
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 HE Rong, LIU Lanqing, L Shengmin, et al. Virology Laboratory, 2nd Clinical Hospital, China Medical University, Shenyang 110004, China;Quantitative detection of HCMV-DNA from urine in infants by FQ-PCR[J];Chinexe Journal of Pediatrics;2001-12
2 HE Rong, RUAN Qiang, JI Yao-hua, LIU Qing, CHEN Shu-rong, LIU Lan-qing, L Sheng-min. Virus Laboratory, 2nd Clinical Hospital, China Medical University, Shenyang 110004, China Corresponding author: HE Rong, E-mail: keren -rong@sina.com;A study on variability of human cytomegalovirus UL144 gene in low-passage clinical isolates[J];Chinese Journal of Microbiology and Immunology;2003-01
3 RUAN Qiang, LU Ying, HE Rong, JI Yao-hua, QI Ying, LIU Qing, CHEN Shu-rong, MA Yan-ping. Virus Laboratory, The 2nd Clinical Hospital, China Medical University, Shenyang 110004, China;Polymorphism of human cytomegalovirus UL136 gene in low passage clinical isolates[J];Chinese Journal of Microbiology and Immunology;2003-09
4 JI Yao-hua, RUAN Qiang, HE Rong, QI Ying, MA Yan-ping, Sun Zheng-rong, LIU Qing, CHEN Shu-rong, ZHOU Yan-li. Virus Laboratory, the 2nd Clinical Hospital, China Medical University, Shenyang 110004, China;Polymorphism of human cytomegalovirus UL149 gene in low passage clinical isolates[J];Chinese Journal of Microbiology and Immunology;2004-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Fang Youbing1,2,Liu Qian1,Wang Mingli1,Shen Ji-jia1.1.Department of Microbiology and Parasitology,Anhui Medical University.2.Department of Laboratory Medicine,the first Affiliated Hospital,Anhui Medical University,Hefei 230032,China.;Diagnosis on HCMV infection in children by Real-time Fluorescence Quantitative PCR for HCMV-DNA detection and cell culture for virus isolation[J];Journal of Tropical Diseases and Parasitology;2008-01
2 SUN Zhengrong~1,JI Yaohua~1,RUAN Qiang~1,XIAO Gengfu~2,HE Rong~1,QI Ying~1,MA Yanping~1 (1.Virus Laboratory,The Second Affiliated Hospital,China Medical University,Shenyang 110004,Liaoning,China;2.College of Life Sciences,Wuhan University,Wuhan 430072,Hubei,China);Genetic Variability of Human Cytomegalovirus UL131A,UL130,UL128 Genes in Strains from Congenitally Infected Infants[J];Journal of Wuhan University(Natural Science Edition);2006-06
3 Yan-Ping Ma,Qiang Ruan,Rong He,Ying Qi,Zheng- Rong Sun,Yao-Hua Ji,Virus Laboratory,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,Liaoning Province,China;Sequence analysis of human cytomeglovirus UL141 A and B genes in strains isolated from infants with jaundice or Hirschsprung’s disease[J];World Chinese Journal of Digestology;2008-13
4 Rong He,Qiang Ruan,Ying Qi,Yan-Ping Ma,Virus Laboratory,Shengjing Hospital of China Medical Univer-sity,Shenyang 110004,Liaoning Province,China;Sequence variability of human cytomegalovirus UL147 genes in clinical isolates[J];World Chinese Journal of Digestology;2009-24
5 Xiao-Bo Yin,Zhi-Qin Mao,Shu-Ting Chang,Mei Sun,Department of Pediatric Gastroenterology,Shengjing Hospital,China Medical University,Shenyang 110004,Liaoning Province,China Yao-Hua Ji,Rong He,Ying Qi,Qiang Ruan,Yan-Ping Ma,Zheng-Rong Sun,Virus Laboratory,Shengjing Hospital,China Medical University,Shenyang 110004,Liaoning Province,China;Sequence polymorphisms of the UL147 gene in clinical strains of human cytomegalovirus[J];World Chinese Journal of Digestology;2012-04
6 ZHANG Li,CAO Chun-Lan,WANG Shu-Jie,et al.Department of Genetics,Women and Children Health Care Institute in Shenyang,Shenyang 110032,Liaoning, China;Study on status of HCMV infection and FQ-PCR detection in infants with jaundice[J];Maternal and Child Health Care of China;2006-24
7 CHEN Wei-Jian,LIU Xiao-Xian.Institute of Pediatric Medicine,Children's Hospital of Hunan,Changsha 410007,Hunan,China;Investigation on partial effect factors of onset of congenital megacolon[J];Maternal and Child Health Care of China;2011-13
8 MA Yan ping,RUAN Qiang, QI Ying, HE Rong,LU Ying,JI Yao hua,LIU Qing, CHEN Shu rong (Virus laboratory,The Second Affiliated Hospital,China Medical University,Shenyang 110004,China);Polymorphism of human cytomegalovirus UL138 gene in low-passage clinical isolates[J];Journal of China Medical University;2004-03
9 QI Ying,RUAN Qiang,MA Yan-ping,HE Rong,JI Yao-hua,SUN Zhengrong,HUANG Yu-jing,LIU Qing,CHEN Shu-rong,WANG Ji-dong (Virus Laboratory,The Second Affiliated Hospital,China Medical University,Shenyang 110004,China);Polymorphism of human cytomegalovirus UL141 gene in clinical isolates of low passage[J];Journal of China Medical University;2005-05
10 SUN Zheng-rong,RUAN Qiang,HE Rong,MA Yan-ping,QI Ying,JI Yaohua(Virus Laboratory,The Second Affiliated Hospital,China Medical University,Shenyang 110004,China);Polymorphism of human cytomegalovirus UL135 gene in low passage clinical isolates[J];Journal of China Medical University;2006-01
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 HE Rong, LIU Lanqing, L Shengmin, et al. Virology Laboratory, 2nd Clinical Hospital, China Medical University, Shenyang 110004, China;Quantitative detection of HCMV-DNA from urine in infants by FQ-PCR[J];Chinexe Journal of Pediatrics;2001-12
2 HE Rong, RUAN Qiang, JI Yao-hua, LIU Qing, CHEN Shu-rong, LIU Lan-qing, L Sheng-min. Virus Laboratory, 2nd Clinical Hospital, China Medical University, Shenyang 110004, China Corresponding author: HE Rong, E-mail: keren -rong@sina.com;A study on variability of human cytomegalovirus UL144 gene in low-passage clinical isolates[J];Chinese Journal of Microbiology and Immunology;2003-01
3 QI Ying, LU Ying, MA Yan ping, RUAN Qiang, HE Rong, JI Yao hua, LIU Qing, CHEN Shu rong. Virus Laboratory, 2nd Clinical Hospital, China Medical University, Shenyang 110004, China;The polymorphism of human cytomegalovirus UL138-UL142 genes in low passage clinical isolates[J];Chinese Journal of Microbiology and Immunology;2004-03
4 Wang Lianying; Li Zheng; RuanQiang;( Pediat.Surg.,2 nd Affiliated Hospital,China Medical University, Shenyang 110003);Congenital Megacolon and Cytomegavirus Infection in Infants[J];CHINESE JOURNAL OF PEDIATRIC SURGERY;1996-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved