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《高分子科学(英文版)》 2016-01
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Effects of Chirality on Gene Delivery Efficiency of Polylysine

Bin-wei Zhao;Zhuxian Zhou;Youqing Shen;Center for Bionanoengineering and State Key Laboratory for Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University;  
Chirality is a key factor in the biological activity of many biomolecules. Poly(L-lysine)(PLL), a polypeptide synthesized from L-lysine, is one of the mostly used cationic polymers for gene delivery. The effect of chirality of polylysine(PL) on its gene delivery remains unknown. Herein, we prepared three polylysines(PLs) with the similar molecular weight but different backbone chiralities including poly(L-lysine)(PLL), poly(D-lysine)(PDL) and poly(DL-lysine)(PDLL). The side chains of each PL were modified with propylene oxide(PO) of different chiralities including(R)PO,(S)PO and(R,S)PO. These PL-POs with distinct chirality in main and side chains could condense p DNA into polyplexes. The polyplexes had approximately the same size, zeta potential and binding ability, but showed distinct gene transfection efficiency. We found that the PLs of L-configuration in the main chain had higher transfection efficiency than that of D or DL configuration due to their faster cellular uptake, while the side chain chirality had no effect on transfection efficiency.
【Fund】: financially supported by the National Basic Research Program(No.2014CB931900);; the National Natural Science Foundation of China(No.21090352)
【CateGory Index】: R943
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【Citations】
Chinese Journal Full-text Database 5 Hits
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【Co-citations】
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3 Zi-xue Jiao;Jie Cen;Hua-yu Tian;Xue-si Chen;Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences;University of Chinese Academy of Sciences;;Synergistic Antitumor Effect of Cisplatin and siRNA Mediated by Polyethylenimine[J];Acta Polymerica Sinica;2015-01
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