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《Chinese Journal of Bone and Joint Injury》 2009-01
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Comparative Study on Main Biological Characteristics of Pre-transfected Rabbit Marrow Stromal Cells and Post-transfected with pcDNA3-hBMP2

Wei Licheng,Liu Danping,Pu Qin,et al.Department of Orthopedics,the First People Hospital,Shaoyang,422000  
Objective To observe main biological characteristic and changes before and after MSCs were transfected with pcDNA3-hBMP2.Methods MSCs from an adult New Zealand white rabbit were isolated and cultivated.MSCs were divided into two groups.Group A was transfected with pcDNA3-hBMP2 and group B was not transfected as control group.Immunohistochemical staining was used for detecting BMP2 expressing 2 days after transfection.The cell morphological changes,cell divide index and cell cycle were observed after transfection.Results Rabbit MSCs could be successfully transfected by pcDNA3-hBMP2 through medium fugene 6 and express BMP2.Primary MSCs proliferated as short spindle shape,while post-transfected MSCs showed triangle-shape,and transfected-cells had significant difference in cell cycle and cell divide index.Conclusion Rabbit MSCs can be successfully transfected by pcDNA3-hBMP2 and after transfection many biological characteristics are significantly changed in this study.
【Fund】: 国家自然基金资助项目(30672125)
【CateGory Index】: R329
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【References】
Chinese Journal Full-text Database 1 Hits
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【Co-references】
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1 TONG Kai, XIE Yi-Jun, WANG Yue-Gang, GUO Shou-Gui, LAI Wen-Yan, WU Ping-sheng Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Construction and identification of adenovirus vector for double-mutant human hypoxia-inducible factor-1α gene[J];Journal of Southern Medical University;2007-04
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8 Wei Li-cheng1, Liu Dan-ping2, Pu Qin31Department of Orthopedics, First People’s Hospital of Shaoyang City, Shaoyang 422000, Hunan Province, China; 2Department of Orthopedics, First Hospital Affiliated to Liaoning Medical University, Jinzhou 121001, Liaoning Province, China; 3Department of Biochemistry, Fourth Military Medical University of Chinese PLA, Xi’an 710033, Shaanxi Province, China;In vitro expression and in vivo osteogenic capability of pcDNA3-hBMP2-transfected marrow stromal cells in rabbits[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-38
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