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《Chinese Journal of Bone and Joint Injury》 2017-08
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Expression of Ihh and PTHrP in Entheses of Achilles tendon of mini pigs

HAN Xue-song;ZHUANG Yan-feng;ZHANG Zhi-hong;WANG Wan-ming;GUO Lin;Department of Orthopedics, Fuzhou Dongfang Hospital, Xiamen University(Fuzhou General Hospital);  
Objective To observe each layer structure and the expression and localization of Col Ⅰ, Col Ⅱ,ColⅩ, Ihh and PTHrP in the Entheses of Achilles tendon of mini pigs. Methods According to the organizational structure of the Entheses,HE staining, Alcian blue staining and Sirius red staining were used to characterize the structure of the entheses. Col Ⅰ, ColⅡ,Col Ⅹ Immunohistochemical staining were used to observe the cartilage extracellular matrix composition. Ihh and PTHr P Immunohistochemical staining were used to observe expression and localization in the entheses. Results The entheses anatomical layer was clear, mainly divided into four layers, fibers tendon, fibrous cartilage layer(also called the Un-calcified cartilage layer), calcified cartilage layer and bone. Extracellular matrix of entheses of Achilles tendon was made up of collagen type Ⅰ, Ⅱ. Ihh and PTHrP immunohistochemical staining was positive, Ihh and PTHrP positive area mainly concentrated in the fibrous cartilage layer and calcification of the Entheses. Hypertrophy of cartilage cell could be observed in cytoplasm.Conclusion The entheses fiber cartilage layer is made up of fibrochondrocytes, it is the important structure of the entheses,Ihh and PTHrP positioning expressed in the fibrous cartilage laye and Un-calcified cartilage layer prompt that they play an important role in regulating in the formation process of entheses.
【Fund】: 南京军区重点课题(ZD35)
【CateGory Index】: R686
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