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《Journal of Arid Land Resources and Environment》 2017-09
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AFLP analysis on genetic diversity of Leymus racemosus in Xinjiang

CAI Xiaoxia;WUMAIERXIATI Tahan;DAI Peihong;JIAERKEN Qiemusen;College of Grassland and Environmental Science,Xinjiang Agricultural University;College of Agriculture,Xinjiang Agricultural University;Xinjiang Grassland Station;  
The floresent amplified fragment length polymorphsim( AFLP) was used to evaluate the genetic diversity and genetic structure of six natural populationsof Leymus racemosus,which is an endangered species with a narrow distribution in Xinjiang,northwest of China. These selective amplification primers generated a total of 1,269 unambiguous bands,of which polymorphic sites is 1,203( 80. 61%). At species level,Nei's gene diversity( H) is 0. 188,and Shannon's information index( I) is 0. 313. At population level,H = 0. 177 and I = 0. 288.Genetic coefficient of differentiation is 0. 1009,which shows 10. 09% genetic variation exists among 6 populations. The gene flow from Fst( Nm) is 4. 4554,which prevents genetic differentiation caused of genetic drift,and genetic differentiation presents homogenization trend. Clustering results showed that a certain correlation exists between populations of L. racemosus genetic and geographic distances. Mantel test showed a positive correlation between geographic distance and genetic distance,but does not reach a significant level( p 0. 05). The study results showed that endangered plant may not be displayed by lower level of genetic variation,it is not only related to the species itself characteristics and research methods,but also on human activities and climatic environment.
【Fund】: 国家自然科学基金项目(31160134)资助
【CateGory Index】: Q943.2
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