Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《国际感染杂志(电子版)(英文版)》 2012-01
Add to Favorite Get Latest Update

Evaluation of Rapid Detection of Nasopharyngeal Colonization with MRSA by Real-Time PCR

Feng-feng Kang;Shu-yan Cao;Yan-ming Li;Jiang-tao Li;Qing He;Yi Li;Yun-jian Hu;Beijing Hospital,Ministry of Health,Institution of Laboratory Medicine,Xiangya School of Medicine,Central South University;Beijing Hospital,Ministry of Health;  
Objective To investigate the clinical application of Real-Time PCR for rapid detection of methicillin-resistant Staphylococcus aureus(MRSA) directly from nasopharyngeal swab specimens.Methods We collected the nasal and throat swab specimens from patients or medical staffs in 3 intensive care units,blood laminar flow ward and respiratory ward in Beijing Hospital,Ministry of Health from December 2010to April 2011.Each sample was tested by RT-PCR and conventional culture-based method for the presence of MRSA.Results The total number of the specimens was 206.Compared with the conventional culture-based method,we demonstrated the diagnostic values for Real-Time PCR were 96.4%sensitivity,96.6%specificity,81.8%positive predictive rate,and 99.4%negative predictive rate.And the limit of detection was 10~2CFU/ml.Conclusions This Real-Time PCR is a simple,rapid,sensitive and specific method.With the high negative predictive value,it can be used for the exclusion of MRSA colonization or infection.However,the application of its low positive predictive value should be further evaluated.
【CateGory Index】: R440
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Xin Ee Tan;Hui-min Neoh;Salasawati Hussin;Noraziah Mohamad Zin;Department of Biomedical Science,Faculty of Allied Health Sciences;UKM Medical Molecular Biology Institute(UMBI);Department of Medical Microbiology and Immunology,Universiti Kebangsaan Malaysia;Programme of Biomedical Science,School of Diagnostic and Applied Health Sciences,Faculty of Health Sciences Universiti Kebangsaan Malaysia;;Clonal distribution and possible micro evolution of methicillin-resistant Staphylococcus aureus strains in a teaching hospital in Malaysia[J];亚太热带生物医学杂志(英文版);2013-03
2 ZHU Lin;ICU of the First People′s Hospital of Zhumadian City in Henan Province;;The analysis of linezolid for the elderly hospital methicillin-resistant Staphylococcus aureus infections[J];China Modern Medicine;2013-32
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved