In vitro activity of allicin combined with two antibiotics on intestinal Shigella
Yuchi Jia;Xiaomei Wu;Department of Infection Research Institute, The Second Hospital of Tianjin Medical University;
Objective: We aimed to evaluate the combined antibacterial effects of allicin in combination with levofloxacin and ceftriaxone on Shigella isolated from the intestinal tract in vitro. Materials and Methods: Using a checkerboard design, broth microdilution assay was used to test the effects of the compounds on the organism. We also determined the MIC of the two groups of antibacterial drugs against 30 strains of Shigella and calculated the fractional inhibitory concentration(FIC) index, to judge the combination effect. Result: After the combined application of allicin and ceftriaxone the MIC decreased significantly. Distribution of the FIC index was as follows: FIC ≤0.5, accounting for 10%; 0.5 FIC ≤1.0, accounting for 60%; 1 FIC ≤2, accounting for 30%; FIC 2, percentage is zero. After combined application of allicin and levofloxacin, distribution of FIC index was as follows: FIC≤0.5, ratio is zero; 0.5 FIC ≤1, accounting for 56.7%; 1 FIC ≤2, accounting for 43.3%; FIC 2, ratio is zero. Conclusion: After the combined use of ceftriaxone, levofloxacin, and allicin, most of the tests showed synergistic effects and additive effects on Shigella, while some of them showed no correlation and no antagonistic effect.
【CateGory Index】： R574.62