Effects of Aflibercept on ion channel of retinal Müller cell membrane cultured in vitro
Qi-Feng Lei;Wei Cai;Department of Ophthalmology,Dongfeng Hospital Affiliated to Hubei University of Medicine;
AIM: To investigate the effects of Aflibercept on the K+channel of retinal M üller cell m em brane cultured in vitro. METHODS: Human Müller cells were divided into 3 groups( control group, high glucose group and experim ental group). The control group were cultured in conventional DM EM m edium; the high glucose group were cultured in high glucose DM EM m edium; the experim ental group were cultured with high glucose DM EM m edium and 100μ m ol/L Aflibercept,and the K+concentration of the cells were detected by M QAE,and the cell survival were detected by M TT assay,the flow cytom etry were used to detect apoptosis rates,Western blot analysis were used to detect the M üller cell caspase-3 protein levels.RESULTS: The Müller cells were positive for glutamine synthetase( GS) after 48 h of culture,and the purification degree were above 90%. The relative concentrations of K+in the control group, high glucose group and experim ental group were( 2. 14± 0. 44) %,( 23.11± 4. 39) %,( 5.20± 0. 92) %,and cell viability were( 100. 00± 0. 00) %,respectively( 73. 24 ± 4. 13) %,( 85. 22 ± 5. 33) %, the apoptosis rates were( 5. 03 ± 1. 91) %,( 26. 73 ± 3. 14) %,( 16. 63± 2. 73) %,and compared the differences between tw o groups were statistically significant( P 0. 05).Com pared with the control group,the level of caspase-3 protein in the high glucose group M üller cells were increased significantly( P0.05); com pared with the high glucose group M üller cells,the caspase-3 protein level in the experim ental group M üller cells were decreased significantly( P0.05).CONCLUSION: Aflibercept can inhibit the K+channel of retinal M üller cells in vitro,inhibit the apoptosis of M üller cells induced by high glucose,decrease the expression of caspase-3 protein,and prom ote cell proliferation.