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《The Chinese Journal of Clinical Pharmacology》 2017-16
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Study on the antifungal activity of Honghefujie lotion against Candida Albicans biofilm and pathogen of superficial mycoses in vitro

JIN Zhen;YAO Feng;SHANG Chen-xu;LI Ji-hong;MO Fei;XIAO Yun-fang;MEI Long;LIN Rong;WANG Wei-rong;Health Science Center,Xi'an Jiaotong University;  
Objective To detect the in vitro antifungal activity of Honghefujie( HHFJ) lotion against Candida Albicans biofilm and superficial fungus. Methods The antifungal activity of HHFJ lotion against Candida Albicans biofilm was investigated by microscopes and crystal violet staining method. The reactive oxygen species( ROS) level in the Candida Albicans biofilm was observed by confocal laser scanning microscopy( CLSM). The minimal inhibitory concentration( MIC) and minimal bactericide concentration( MBC) with superficial fungus were determined by the microporous double dilution method. The bactericidal rate was calculated through colony counting method. Results The results showed that HHFJ lotion after 40-fold dilution was effective in inhibiting Candida Albicans biofilm formation. HHFJ lotion under 10-fold dilution could eliminate mature biofilms. HHFJ lotion elevated ROS levels in biofilms.Besides,HHFJ lotion was active against almost tested superficial fungus,the MIC was almost 32-128 fold dilution and the MBC was almost 16-32 fold. The original concentration,5 and 10 times diluents of HHFJ lotion had strong antifungal effect on the tested fungus.Conclusion HHFJ lotion has good antifungal activity in vitro against Candida Albicans biofilm and superficial fungus.
【CateGory Index】: R285
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