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《The Chinese Journal of Clinical Pharmacology》 2017-16
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Expression and significance of cytochrome P4502E1 of alcoholic liver injury in mice

QIAO Xin-jie;LI San-qiang;HUO Xu-lei;LI Xiao-ping;ZHANG Yi-jiong;SUN Hai-kuan;WANG Shan-long;ZHANG Yong-yong;LU Qing;MENG Fan-zhu;Key Laboratory of Hepatic Injury and Restoration, Medical College of Henan University of Science and Technology;  
Objective To study the dynamic changes of cytochrome P4502E1( CYP2E1) in alcohol-induced liver injury in mice and its significance. Methods Fifty male mice were randomly divided into model group( n = 40) and control group( n = 10). The model of alcoholic liver injury was established by continuous infusion of 56 ° Erguotou at 10 mL·kg~(-1)for 4 weeks. At the time of 1,2,3,4 weeks,to take 10 mice,eye blood for serum aspartate aminotransferase( AST) activity determination. Followed by cervical dislocation of mice,the number of CYP2E1 positive cells in mice liver were detected by immunohistochemical SP method. Results The activity of hepatic AST enzyme in model group were( 126 ± 24),( 967 ± 30),( 1010 ± 35) and( 206 ± 23) U·L~(-1)in1 st,2nd,3rd and 4th weeks,respectively. Compared with the control group( 112 ± 22) U · L~(-1),the activity of hepatic AST enzyme in the model group increased continuouslyfrom 1st to 3rd weeks and reached the peak at 3rd week with significantly( P 0. 01),which was decreased at the 4th week,but still higher than the control group with significantly( P 0. 05). The number of CYP2E1 positive cells per square millimeter were( 3. 2 ±0. 8),( 8. 4 ±1. 1),( 13. 2 ±1. 3),and( 4. 6 ±0. 8) cell·mm~(-2)in 1st,2nd,3rd and 4th weeks. Compared with the control group( 2. 8 ± 0. 5) cell·mm~(-2),the number were obviously increased in 1st,2nd and 3rd weeks and represented statistically significant( P 0. 01) while the number of the 4th week decreased but still with statistically significant( P 0. 05). Conclusion The expression of CYP2E1 was increased first and then decreased in alcohol-induced liver injury model,which was consistent with the trend of serum AST enzyme activity. The dynamic changes in expression may be associated with damage and repair of the liver.
【Fund】: 国家自然科学基金资助项目(81201558);; 河南省科技攻关基金资助项目(172102310693);; 河南科技大学创新团队基金资助项目(2015XTD003)
【CateGory Index】: R575
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