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《The Chinese Journal of Clinical Pharmacology》 2017-16
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Effects of microRNA-19b and microRNA-185 on proliferation and apoptosis of laryngeal squamous cell carcinoma

LI Kai;BAO Han;ZHANG Pan-ying;CHEN Li;Department of Otorhinolaryngology,Xi'an Ninth Hospital;  
Objective To explore the functions of microRNA( miR)-19b and miR-185 on proliferation and apoptosis of laryngeal carcinoma cells.Methods The laryngeal squamous carcinoma cell line( Hep-2 cell) as control group, construction the miR-19b and miR-185 deletion mutants by the technology of Crisp-Case 9 as miR-19b and miR-185 deletion groups,and building miR-19b and miR-185 overexpression mutants by cell transfer dyeing technology as miR-19b and miR-185 overexpression groups. Flow cytometry was used to detect the apoptosis of control group,miR-19b,miR-185 deletion groups and miR-19b,miR-185 overexpressing groups. The apoptosis of laryngeal carcinoma cells in five groups was detected by flow cytometry. The proliferation of Hep-2 cell lines( expressed by OD_(450 nm)) and the invasive ability( expressed as the number of invasive cells) were measured underdifferent conditions. Results After 64 h culture,the proliferation ability of miR-19b and miR-185 overexpression groups and miR-19b,miR-185 deletion groups were 0. 65 ± 0. 01,0. 63 ± 0. 01,1. 69 ± 0. 03,1. 73 ± 0. 02,had significant difference with that in control group,which was 1. 28 ± 0. 02( P 0. 01). The apoptosis of miR-19b and miR-185 overexpression groups and miR-19b, miR-185 deletion groups were( 12. 32 ± 1. 39) %,( 11. 74 ± 1. 08) %,( 3. 28 ± 0. 11) %,( 3. 48 ± 0. 12) %,had significant difference with that in control group,which was( 5. 34 ± 1. 27) %( P 0. 01). The number of cells in over expression of miR-19b and miR-185 overexpression groups and miR-19b,miR-185 deletion groups were 12 ± 2,9 ± 3,47 ± 7,49 ± 8,had significant difference with that in control group,which was 36 ± 5( P 0. 05). Conclusion miR-185 and miR-19b can significantly inhibit the proliferation and invasion of laryngeal squamous cell carcinoma.
【Key Words】: laryngeal squamous cell carcinoma cell proliferation cell invasion Hep-2 cell cell culture
【CateGory Index】: R739.65
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