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《The Chinese Journal of Clinical Pharmacology》 2017-16
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Effect of microRNA-23a on proliferation and invasion in breast cancer cells

JIN Ke;ZHU Jian-mei;QIU Fa-qi;Department of Oncology, Sichuan Academy of Medical Sciences,East Department of Sichuan Provincial People's Hospital;  
Objective To investigate the effect of microRNA-23a( miR-23a) on proliferation and invasion of breast cancer cells.Methods Recombinant human breast cancer MCF-7 and MDA-MB-435S were inoculated into 6-well plates and randomly divided into six groups,RNAi Ⅰ group,over expression-Ⅰ group,control Ⅰ group,RNAi Ⅱ group,over expression Ⅱ group,control Ⅱgroup. Construct miR-23 a recombinant interference and over expression vector. That were transfected into MCF-7 and MDA-MB-435S cell lines with lipofectamine 2000. Results OD492 value of MCF-7 cells and MDA-MB-435 S cells after 72 h culture: in over expression group could reach 0. 52 ± 0. 03 and 0. 44 ± 0. 03 respectively while they in RNAi group were only 0. 36 ± 0. 02 and 0. 31 ± 0. 02. Compared with control Ⅰ group and control Ⅱ group,RNAi-Ⅰ group and RNAi-Ⅱgroup had significantly different( all P 0. 05). The doubling time in over expression-Ⅰ and Ⅱ groups were( 26. 61 ± 5. 23),( 29. 23 ± 5. 51) h, respectively, while those in RNAi-Ⅰ and RNAi-Ⅱ groups were( 42. 24 ± 3. 47),( 58. 17 ± 5. 34) h. Theproportion of M phase cells in the over expression Ⅰ and Ⅱ groups were 52. 36%,51. 59%,respectively,while those in RNAi-Ⅰ and RNAi-Ⅱ groups were 76. 70%,74. 17%. Compared with control Ⅰ group,the doubling time and proportion of M phase cells in RNAi-Ⅰ group and over expression-Ⅰ group with significantly( all P 0. 05).Compared with control Ⅱ group,the doubling time and proportion of M phase cells in RNAi-Ⅱ group andover expression-Ⅱ group with significantly( all P 0. 05). Conclusion The expression levels of miR-23 a showed a positive correlation with proliferation and invasion of breast cancer cells.
【Key Words】: breast cancer microRNA-23a proliferation invasion
【Fund】: 四川省卫生和计划生育委员会基金资助项目(140082)
【CateGory Index】: R737.9
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