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《The Chinese Journal of Clinical Pharmacology》 2017-16
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Determination the cotents of oleanolic acid in Hemsleya sinesis Cogn. from different localities by HPLC-evaporative light scattering detector

QIN Xiao-dong;LUO Xian-qin;LI Qing;DENG Ting;HU Rong;Department of Pharmacy,Chongqing Hospital of Traditional Chinese Medicine;Institute of Chinese Pharmacology And Toxicology, Chongqing Academy of Chinese Materia Medica;Institute of Chinese Medicinal Chemistry, Chongqing Academy of Chinese Materia Medica;Drug Review Section,China Chongqing Technical Center for Drug Evaluation and Certification;  
Objective To develop a high performance liquid chromatography-evaporative light scattering detector( HPLC-ELSD) method for the determiantion of oleanolic acid in Hemsleya sinesis Cogn. from different localities. Methods The analysis was carried out with Kromasil C_(18)( 4. 6 mm × 250 mm,5 μm) column at a column temperature of 35 ℃ and the mobile phase consisted of methanol-water-acetic acid( 270∶ 30∶ 0. 2) at a flow rate of 0. 6 mL·min~(-1). The drift tube temperature for the ELSD was set at 85 ℃,and the flow rate of air was 2. 70L·min~(-1). Results The calibration curves were linear within the range of 0. 11-1. 70 μg( y = 23. 11 x + 14. 90,r = 0. 999 9). The average recovery was 99. 18%,RSD = 0. 78%. The highest content of Wuyanling produced in Zhejiang is up to( 0. 18 ± 0. 05) %. The lowest content produced in Guangxi Longzhou is( 0. 08 ± 0. 02) %. Conclusion There were some differences in the contents of oleanolic acid in Hemsleya sinesis Cogn. from different localities because of environmental factors.
【Key Words】: Hemsleya sinesis Cogn oleanolic acid high performance liquid chromatography-evaporative light scattering detector
【Fund】: 重庆市科技研发平台项目基金资助项目(cstc2015pt-nsjg10003);; 重庆市中医管理局2015年中医药部门公共卫生专项基金资助项目
【CateGory Index】: R284.1
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