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《The Chinese Journal of Clinical Pharmacology》 2017-16
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Determination the contents of quercetin and kaempfer in Camptotheca acuminata Decne leaves by HPLC

FAN Hong-mei;LIU Zhi-hai;LIU Yu-ming;L Lei;LONG Lin;ZOU Lan;Qianxinan Institution for Food and Drug Inspection and Control Center;  
Objective To establish a HPLC to analyze the content of quercetin and kaempfer in the Camptotheca acuminata Decne leaves.Methods The sample of Camptotheca acuminata Dene leaves were obtained by 5% hydrochloric acid-methanol. The content of quercetin and kaempferol was measured by HPLC with chromatographic column: Hubble C_(18)( 4. 6 mm × 250 mm,5 μm),mobile phase: CH_3OH-0. 2%H_3PO_4( 30∶ 70),flow rate: 1 mL·min~(-1),column temperature: 30 ℃,detection wavelength: 370 nm. The specificity,standard curve and limit of quantification( LOQ),precision and recovery,stability and repeatability were measured. Results The quercetin and kaempferol existed a good linear relation at 5. 53-69. 08 μg · mL~(-1)and 3. 99-49. 90μg·mL~(-1),respectively. The LOQ of quercetin and kaempferol was 4. 23 and 7. 81 ng,respectively. And the average spotting recovery rate of quercetin and kaempferol was 98. 94% and 98. 29%,respectively. The RSD values of inter-day and intra-day assays were lower than 0. 89% and 1. 04% for quercetin and kaempferol,respectively. Conclusion This method is an accurate,reliable and convenient method,which is suitable for the quality control of the leaves of Camptotheca acuminata Decne.
【Key Words】: Camptotheca acuminata Decne leaf quercetin kaempferol HPLC
【Fund】: 贵州省食品药品监督管理局基金资助项目(DBXZYC52032)
【CateGory Index】: O657.72;R284.1
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