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《Journal of Fruit Science》 2004-01
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AFLP Analysis of the Genetic Diversity Between Diploid and Autopoly-ploidy Watermelon

Liu Wenge 1 ,Wang Ming 2 ,and Yan Zhihong 1 ( 1 Zhengzhou Fruit Research Institute,CAAS,Zhengzhou,Henan450009China ;2 Horticultural College,Northwest Science and Tech nology University of Agric ulture&Forestry,Yangling,Shaanxi712100China)  
Analysis was c on ducted with different ploidy watermelon materials,including diploid,triploid crossed with4x×4x and autotetraploid induced by colchicines of Mimei and JM w atermelon varieties by using AFLP molecular markers.Re-sults showed that using amplified fragment length polymorphism(AFLP)could identify the genetic varia tion of watermelon.We employed48AFLP primer combinations on different ploidy ,a total of4800~5000AFLP bands were observed.AFLP bands of different ploidy watermelon amplified by most AFLP primer pairs were not apparently diffe rent.It indicated low ge-netic variation among4x,3x,2x.3bands of Mimei' s and7bands of JM's were polymorphic,there were1specific band in M2x,1spec ific band in M3x and1specific band in M4x when compared with its other ploidy .There were2specific bands in JM2x,2specific bands in JM3x and3specific b ands in JM4x when compared with its other ploidy.There were present or ab-se nt of specific bands of autotetraploid compared with diploid,this reflected th at DNA molecular genetic structure was changed by colchicines and allelic variat ion of autotetraploid.There were present or absent of specific bands of tripl oid F 1 compared both parents,this reflected that gene mutation occurred in the fer tilization process after cross of the tetraploid and diploid.
【Fund】: 国家863计划子课题项目(No.2001AA241173);; 河南省自然科学基金项目(No.0211030900)
【CateGory Index】: S651
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