Trends of ethylene-regulated gene expression in Citrus reticulata Blanco by microarray analysis
LI Hai-tao1,2,LIANG Guo-lu1 (1College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716 China; 2 Sichuan Foreign Languige College,Shapingba,Chongqing 400031 China)
The continual trend of ethylene-regulated gene expression in mature fruit abscission zones of Citrus reticulata Blanco was investigated with 6 expressed sequence tag-based microarrays. Comparing expression profiles of the ethylene-treated wild-type plants with untreated ones identified ca. 7.26% of the about 30302 probe sets as ethylene-regulated. Increased expression and low expression were detected in RNA of mature fruit abscission zones 4,12 and 24 h after application of ethylene. Ethylene-regulated genes involved in its own biosynthesis and signal transduction pathway were identified. A large number of transcription factors and some putative signaling components were highly regulated by ethylene. Ethylene appeared to regulate other primary metabolic genes. Plant defense and PR protein genes were differentially regulated,with some genes within this class highly up-regulated. The continual trends of 500 genes' expression were found for the first time.