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Effects of ABA on expression of genes related to anthocyanin biosynthesis in grapevine

YU Miao1,3,LIU Hai-feng4,WANG Jun1,2(1College of Forestry,Northeast Forestry University,Harbin,Heilongjiang 150040 China;2Center for Viticulture and Enology,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083 China;3Testing and analysis Center for Agricultural Product,Heilongjiang Academy of Land Reclamation Sciences,Jiamusi,Heilongjiang 154007 China;4Agricultural college of Yanbian University,Yanji,Jilin 133000 China)  
The Jingyou grape cultivars was used to study changes of anthocyanin contents,the transcription of anthocyanin biosynthesis enzyme genes and transcription factors during the fruit coloring period as well as the influence by ABA treatments of different concentrations.The results showed that the transcription of CHS3,CHI2,UFGT,MybA1 and MybA2 genes was closely related to the anthocyanin synthesis during the fruit coloring period and their expression increased as anthocyanin synthesis.Anthocyanin contents increased by the ABA treatment.Meanwhile,the transcription levels and expression time of anthocyanin biosynthesis relevant genes were also improved.The expression of CHS3 and UFGT was significantly improved by ABA.Correlation analysis showed that anthocyanin contents was significantly positively correlated with the expression of some structural genes(CHS3,CHI2,F3H2 and UFGT) and transcription factors(MybA1),while there was no significant correlation between anthocyanin contents and the expression of CHS1,CHS2,CHI1,F3H1,DFR,F3'H,LDOX and OMT.
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