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Studies on highly frequent and efficient plant regeneration system of sweet cherry tetraploid rootstocks Y1

WEI Hai-rong,ZONG Xiao-juan,WANG Jia-wei,LIU Qing-zhong(Shandong Institute of Pomology,Tai'an,Shandong 271000 China)  
In vitro leaves of sweet cherry tetraploid rootstocks Y1 were used as explants to investigate the effect of different basic media,hormone combinations,dark incubation time and leaf physiological status on inducing adventitious shoots regeneration.The objective of this research was to establish a highly frequent and efficient regeneration system of sweet cherry tetraploid rootstocks Y1 so as to make preparation for genetic transformation.The results showed that WPM medium was the optimal basic culture medium for the adventitious shoots regeneration.The effect of the adventitious shoot regeneration in WPM medium was obvious superior to MS medium and DKW medium.The optimal hormone combinations were 6-BA 2.0 mg·L-1+IBA 1.0 mg·L-1.Dark incubation could improve the regeneration rate obviously.The optimum dark incubation time was 14 days.The best young explanding leaves in vitro with the best physiological status for adventitious shoots regeneration.A highly frequent and efficient regeneration system of sweet cherry tetraploid rootstocks Y1 was successfully established.Adventitious shoots regeneration rate was 90% and mean number of adventitious shoots per leaf was 4.1.
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