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Influence of eight citrus cultivars on the composition of Citrus tatter leaf virus

LIU Ke-hong,SONG Zhen,ZHOU Yan,LI Zhong-an,ZHOU Chang-yong(Citrus Research Institute,Southwest University,Beibei,Chongqing 400712 China)  
Citrus tatter leaf disease caused by Citrus tatter leaf virus(CTLV) is an economically important systemic disease of citrus in the world.It is known that CTLV is widespread in China,and it has caused tremendous economic loss in Zhejiang,Hunan,Fujian and Guangxi provinces.So it is important to understand the influence of citrus cultivars on the composition of CTLV.48 sub-isolates of CTLV isolates HH and XLB were obtained by inoculating HH and XLB to Jincheng,Lemon,Ponkan,Tangerine,Daidai,None,Fragrant orange and Rusk citrange.Then restriction fragment length polymorphism(RFLP),single-strand conformation polymorphism(SSCP) and sequence analysis were applied to analyze the variation of HH,XLB and their sub-isolates.The results showed that the RFLP patterns of coat protein gene(CP) digested by restriction enzyme Hinf I of CTLV isolate HH,XLB and their sub-isolates were similar.Based on SSCP analysis,HH mixed with several strains changed its polymorphism in some hosts.However,XLB with single strain did not change its polymorphism in different citrus hosts.In sequence analysis,it was found that the nucleotide identity in the CP gene of isolate HH and its sub-isolates was 99.3% to 100%,and that of isolate XLB and its sub-isolates was 96.5% to 99.8%.The results indicated that the composition of CTLV isolates might be changed in different citrus hosts.
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