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《JOURNAL OF FRUIT SCIENCE》 1996-01
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Cloning and Expression of Coat Protein Gene of Watermelon Mosaic Virus 2 in Escherichia coli

Wan Li; Liu Junjun; Peng Xuexian and Huang Xuesen(Zhengzhou Fruit Research Institute,CAAS,Henan 450004,Institute of Microbiolegy,Academia Sincia)  
An isolate was separated from infected watermelon leaves and then identified the watermelon mosaicvirus 2.cDNA was synthesized by reverse transcription by using viral RNA as the template through PCR(poly-chain-reaction).The coat protein gene(CP),including 3' non-coding region,was amplified andcloned.The complete sequence of nucleotides was established.The full length of CP gene is 1099 nucleotidesincluding 903 nucleotides in the region coding,301 amino acids in each encode,and 196 nucleotides in the 3'untranslated region。The coding region of WMV-2CP gene is 60 nucleotides and 20 amino acids longer thanthat of A-WMV 2and U- WMV-2strain。Comparing the later strain,the homology nucleotide se-quence in the coding region are 88.5%and 87. 0%,respectively; the homology of the amino acid sequenceare 90.0%and 88.7%,respectively;and the homology of3'non-coding sequence are 68.3%and 71.8%,respectively.If the last 60 nucleotides and 20 amino acids in WMV-2CP gene are not taken into account,thehomology mentioned above will be 95.4%, 93.7%;96.4%,95.0%; 88.8%and 93.4%,respectively。Theexpression vector for WMV- 2CP gene in Escherichia coli was constructed.By the method of SDS- PAGEanalysis,it showed that the MW of the produced gene expressed in E. cori is equal to that of authentic viralcoat protein.
【Fund】: 国际科学和文化中心世界实验室(WL,ICSC,日内瓦、洛桑)的部分资助
【CateGory Index】: S436.5
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