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Isolation,Purification of Genomic DNA and Identification of RFLP in Vitis

Zhang Liping; Lin Bonian; Shen Dexu; and Dong Jixin(Department of Horticulture,Zhejiang Agricultural University,Hangzhou,310029)Nami Yamammoto; Katsumi Hasmzume,andNorihkoUmeda(National Research Institute of Brewing,Japan)  
Genomic DNA had been isolated from young grapevine leaves according to Jennifer and Paul's modifiedmethod and purified by sepharose 2B chromatography,and then successfully used in RFLP analysis.Resultshowed that the combination of PVvc一A/HindⅢ was an effective method used in identifing all tested speciesand cultivars by polymorphism.
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