Isolation,Purification of Genomic DNA and Identification of RFLP in Vitis
Zhang Liping; Lin
Bonian; Shen Dexu; and Dong Jixin(Department of Horticulture,Zhejiang Agricultural
University,Hangzhou,310029)Nami Yamammoto; Katsumi Hasmzume,andNorihkoUmeda(National
Research Institute of Brewing,Japan)
Genomic DNA had been isolated from young
grapevine leaves according to Jennifer and Paul's modifiedmethod and purified by sepharose 2B
chromatography,and then successfully used in RFLP analysis.Resultshowed that the combination
of PVvc一A/HindⅢ was an effective method used in identifing all tested speciesand cultivars by
polymorphism.
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