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《Journal of Gansu Agricultural University》 2005-01
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Plant regeneration from cotyledon and hypocotyl explants of pepper in vitro and high efficient system establishment of plant regeneration

LONG Feng,ZHANG Jin-wen (College of Agronomy, Gansu Agricultural University, Gansu, Lanzhou 730070, China)  
Cotyledon and hypocotyl explants from 9 cultivars of pepper (Capsicum annuum L.) were cultured on MS medium supplemented with B5 vitamins, and different combination of growth regulators.The effects of seedling stage, genotypes, different explant types, combinations of hormone and AgNO3 on differentiation and elongation of shoots of pepper cotyledon and hypocotyl were observed. The results showed that seedling stage had direct effect on the way of shoots differentiation. AgNO3 signifitantly increased the frequency of shoot induction and shortened the time for explant regeneration. The frequency of shoot differentiation from cotyledon explants was higher than that from hypocotyl explants. B5 vitamins were favourable for shoots growth and increasing the frequency of elongation of shoot of pepper explants.The optimal regeneration mediums for shoots induction, shoots elongation and rooting of cotyledon and hypocotyl explants of pepper were obtained: the shoot induction medium: MB5+5 mg/L6-BA+0.5 mg/L IAA+4 mg/L AgNO3; the shoots elongation medium: MB5+3 mg/L 6-BA+1 mg/L IAA+2 mg/L GA3+4 mg/L AgNO3; the rooting medium: 1/2 MS+0.2 mg/L IAA+0.1 mg/L NAA, respectively.
【Fund】: 国家自然科学基金项目(编号:30270843)
【CateGory Index】: S641.3
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Chinese Journal Full-text Database 9 Hits
1 SUN Li-ping et al(Binzhou Vocational College,Binzhou,Shandong 256603);Establishment of Regeneration System from Cotyledon Explant of Pepper and Screening Test for its Resistance to Kanamycin[J];Journal of Anhui Agricultural Sciences;2007-33
2 Hu Nengbing1,2,Yuan Yi1*,Zhang Yang1(1.School of Life Science,Anhui Agricultural University,Hefei,230036; 2.School of Plant Science,Anhui Science and Technology University,Fengyang 233100,Anhui,China);Study of different hormone mixtures and sugar densities on ex-situ regeneration of the purple pepper[J];Forest By-Product and Speciality in China;2008-02
3 HE Xiao-qing,GONG Zhen-hui (College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China);In vitro Regeneration and Its Influential Factors in Pepper (Capsicum annuum L.)[J];Acta Botanica Boreali-Occidentalia Sinica;2007-07
4 HE Jian-wen(Guizhou Pepper Institute,Zunyi,Guizhou 563000,China);Tissue Culture of Pepper and Its Application in Breeding[J];Guizhou Agricultural Sciences;2009-07
5 QIAN Jin1,LIU Fa-yang1,XIE Xiao-dong2,JIA Biao1(1.College of Prataculture,Gansu Agricultural University,Lanzhou 730070;2.College of Life Sciences,Lanzhou University,Lanzhou 730000,China);Establishment of high frequency regeneration system in Alfalfa(Medicago sativa L.)[J];Journal of Gansu Agricultural University;2007-01
6 QIU Cui~1,HE Shui-lin~2 (1.College of Horticulture;2.College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Progress in the construction of in vitro regeneration system of pepper and its application[J];Subtropical Agriculture Research;2006-04
7 HUANG Li-hua1,HONG Ya-hui1,DAI Xiong-ze2,ZHANG Xue-wen1 (1.College of Bioscience and Biotechnology,HNAU,Changsha 410128,China;2.Vegetable Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China);Effect of light quality on calluses induction and differentiation of Capsicum annuum[J];Journal of Hunan Agricultural University(Natural Sciences);2009-06
8 Liu Juan, Tang Haoru, Wang Xiaorong, Luo Ya (College of Forestry and Horticulture, Sichuan Agricultural University, Ya’an, Sichuan 625014);Advances on Application of AgNO_3 in in vitro Culture[J];Chinese Agricultural Science Bulletin;2007-10
9 Mohammad Daoud Eliassi,SHEN Huo-lin,YANG Wen-cai,TAN Fang,WANG Shuai,GUO Shuang(College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China);In Vitro Plant Regeneration and SSR Detecting of Regenerated-plants in Pepper(Capsicum annuum L.)[J];China Vegetables;2009-18
China Proceedings of conference Full-text Database 1 Hits
1 YAN Xiao-jie~1 SHANG Ai-qin~2 ZHAO Liang-jun~1 (~1 Department of Ornamental Horticulture and Landscape Architecture,China Agriculture University,Beijing 100094; ~2 College of Horticulture,Agricultural University of Hebei,Baoding 071001);Adventitious Bud Regeneration from Hypocotyls of Euonymus fortunei[A];[C];2007
Chinese Journal Full-text Database 5 Hits
1 ZHANG Jian-quan1,ZHANG Jin-wen2 (1. Gansu Grassland Ecological Institute, Lanzhou 730020, China;2. Agronomy Academy, Gansu Agricultural University, Lanzhou 730070, China);Methods of transformation of AcInv gene into potato plants mediated by Agrobacterium tumefaciens[J];Journal of Gansu Agricultural University;2002-02
2 Cao Dongsun and Jia Shirong (Biotechnology Research Center , Chinese Academy of Agricultural Sciences, Beijing 100081);In Vitro Plant Regeneration of Sweet Pepper[J];Acta Horticulturae Sinica;1993-02
3 Yu Xiaolin 1,Li Naijian 2,Huang Ziran 1,and Li Ying 2 (1Department of Sericulture South China Agricultural University,GuangZhou 510642; 2Vegetable Research Institute Guangdong Academy of Agricultural Sciences,GuangZhou 510640);In Vitro Shoot Differentiation from Cotyledon Explants of Pepper(Capsicum annuum L.)[J];ACTA HORTICULTURAE SINICA;2000-01
4 Li Yinghui,Li Yan,Hang Xiaoming,and Wang Peng (Key laboratory of Liaoning Province for Plant Biotechnology,Liaoning Normal University,Dalian 116029);Effects of Cytokinins on Shoot Organogenesis from Cotyledon of Pepper[J];Acta Horticulturae Sinica;2001-03
5 Li Dingjun 1,2 , Zhang Baoxi 1, Zhao Kaijun 1,Xie Bingyan 1, and Luo Kuan 2 ( 1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Science, Beijing 100081; 2Department of Plant Protection, Hunan Agricultural University,Changs;High Efficient System Establishment of Plant Regengation from Cotyledon Explants of Pepper( Capsicum annuum L.)[J];Acta Horticulturae Sinica;2002-01
Chinese Journal Full-text Database 10 Hits
1 Zhang Hua et al (Xuzhou Sweet Potato Research Center, Xuzhou 221121);Studies on the Techniques and Methods of Thousands of Head Chrysanthemum Stem Tip Induction and Plantlet Regeneration[J];Journal of Anhui Agricultural Sciences;1999-06
2 MA Li-zhen (Faculty of Agriculture and Animal Science,Qinghai University, Xining,Qinghai 810003);Research on Browing in Plant Tissue Culture[J];Journal of Anhui Agricultural Sciences;2006-15
3 SUN Li-ping et al(Binzhou Vocational College,Binzhou,Shandong 256603);Establishment of Regeneration System from Cotyledon Explant of Pepper and Screening Test for its Resistance to Kanamycin[J];Journal of Anhui Agricultural Sciences;2007-33
4 WANG Ping et al(School of Marine Science and Techology,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005);Study on Sensibility of the Potato Cultivar Dongnong 303 to Two Kinds of Antibiotics[J];Journal of Anhui Agricultural Sciences;2008-16
5 LI He-chun et al (College of Life Science,Northeast Agricultural University,Harbin,Heilongjiang 150030);Discussion on the Tissue Culture and Rapid Propagation Techniques of Clerodendrum thomsonae Balf.[J];Journal of Anhui Agricultural Sciences;2009-21
6 CHEN Yong-bo,ZHAO Qing-hua (The Konjac Research Institute of Enshi Autonomic State Academy ofAgricultural Sciences , Enshi 445000, China);Disinfection Technology for Young Konjac in Test Tube[J];Amino Acids & Biotic Resources;2005-03
7 WANG Qian-fang(Yellow Slightly Agricultural Technology Extension Station,Suixi County Bureau of Agriculture,Zhanjiang Guangdong 524335);Study on Cotyledon culture of Bulgaria Capsicum in Vitro and Establishment of Plant Regeneration[J];;2009-11
8 LI Cheng-wei,YAO Xiao-hui,CHEN Xin-bing(Department of Live Science,Shangqiu Normal University,Shangqiu,Henan 476000,China);Effect of 2,4-D and 6-BA on Callus Induction of Helianthus tuberosus Linn[J];Northern Horticulture;2008-02
9 ZHANG Xue-ying,LIU Yan-meng,HU Shu-ming ZHANG Gang(Agricultural University of Hebei,Baoding,Hebei 071001,China);Study on the Tissue Culture of Betula platyphylla Suk.[J];Northern Horticulture;2008-08
10 LI Hong-shuang,LIU Qiao-hui(College of Environmental Science and Engineering,Nanjing University of Information Science and Technology,Nanjing,Jiangsu 210044,China);Study of Nutrients Absorption During Plant Growth[J];Northern Horticulture;2008-12
China Proceedings of conference Full-text Database 3 Hits
1 Shen Huolin, Wang Zhiyuan & Jiang Jianzhen (Dept. of Horticulture, Beijing Agricultural University, Beijing 100094);In vitro plant regeneration and variation of pepper[A];[C];1994
2 Huang Wenjiang Liu Qingzhong College of Horticulture, Northwestern Science and Technology University of Agriculture and Forestry, Yangling Shaanxi,712100; Shandong Institute of Pomology,Tai' an,Shandong 271000);Advance of Plant Regeneration from Perennial Fruit Crop's In Vitro Leaf[A];[C];2001
3 Guo Wenguang Bai Ronglin Cheng zhuomin Li Shifang (The Quartermaster Univeoity of PLA, Changchun, 130062; The State Key Lab for Biology of Plant Disease and Insect Pest, Institute of Plant Protection, CAAS, Beijing 100094);Research Progress on Tissue Culture and Genetic Transformation of Pepper[A];[C];2004
Chinese Journal Full-text Database 10 Hits
1 Chen Jingxian;Nie Fan, (Institute of Horticulture, Anhui Academy of Agricultural Sciences);STUDIES ON THE COTYLEDEN CULTURE AND PLANT REGENERATION OF HOT-PEPPER[J];Journal of Anhui Agricultural Sciences;1990-03
2 SUI Yi - hu, ZHANG Zi - xue, XING Su - zhi, LING Tong - zhong, JIN Gui - fang (Department of Agronomy,Auhui Technical Teachers College,Fengyang 233100,China);Research on Some Physiologic Indexes and Germination of Purple Chili Line YN99007 under NaCl Stress[J];Journal of Anhui Technical Teachers College;2004-06
3 SUI Yi-hu1,2,ZHANG Zi-xue1,HU Neng-bing1,WANG Xia1(1.College of Plant Science,Anhui Science and Technology University,Fengyang 233100,China;2.The State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210094,China);Changes of some Quality Indexes in the Process of Purple Chili mix-pickled with Cucumber or Mustard[J];Journal of Anhui Science and Technology University;2007-06
4 LI Hua-ping 1, John S Hu 2, WANG Ming 2, FAN Huai-zhong 1 (1. South China Agricultural University, Guangzhou 510642, China; 2. University of Hawaii, USA 96822);Studies on Transgenic Pepper Plants Transferred with the Coat Protein Gene of Cucumber Mosaic Virus[J];CHINESE JOURNAL OF VIROLOGY;2000-03
5 ZHANG Li-yi~(1), GAO Xia-hong~(2),YU Tong-quan,WANG You-nian~(2) (~1Inner Mongolia Agricultural University, Hohhot 010018;~2Beijing Agricultual College,Beijing 102206);Study on Regeneration System for Strawberry in vitro[J];Journal of Beijing Agricultural College;2004-02
6 YANG Qi-jian~1, ZHOU He~2, SUN Yan~2, LU Ping~1 (~1 Beijing Agricultural College, Beijing 102206;~2China Agricultural University, Beijing 100094 );Study on Induction Callus of Alfalfa and Its Tissue Culture[J];Journal of Beijing Agricultural College;2004-02
7 Bie Zhilong; Liu Peiying;In Vitro Culture and Genetic Transfermation in Pepper[J];Journal of Changjiang Vegetables;1996-08
8 Dong Yanrong )Gong Yiqin;Progress of Anther and Pollen Culture of Solanum Vegetable Crops[J];Journal of Changjiang Vegetables;2001-05
9 Zhou Xuefei,Liu Chunsong,Tang Maoju,Yuan Guishi;Tissue Culture of Pepper Cotyledon[J];Journal of Changjiang Vegetables;2001-07
10 Deng Minghua,Zou Xuexiao,Zhou Qunchu,Liu Zhimin,Wen Jinfen,Du Feng;Study on Plant Regeneration of Cotyledon Culture of Capsicum[J];Journal of Changjiang Vegetables;2003-06
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 QI Wei-qiang et al(Forestry and Horticulture College,Henan Agriculture University,Zhengzhou,Henan 450002);Research Progress in the Eggplant Anther and Pollen Culture Technology[J];Journal of Anhui Agricultural Sciences;2008-12
2 QU Jing et al(Biotechnology Center of Jilin Agricultural University,Changchun,Jilin 130118);Optimization of the Regeneration Conditions from Alfalfa Hypocotyls[J];Journal of Anhui Agricultural Sciences;2009-19
3 CHAI Yan-wen1,MA Hui-ling1,XIE Xiao-dong2,LIU Yong-cai1(1.College of Pratacultural Science,Gansu Agricultural University,Lanzhou 730070,China;2.College of Life Sciences,Lanzhou University,Lanzhou 730000,China);Effects of phytohormones screening on callus induction of alfalfa with orthogonal design[J];Grassland and Turf;2008-05
4 LI Fenglan, LIU Rongmei, HU Guofu, HU Baozhong (College of Life Sciences, Northeast Agricultural University, Harbin 150030, China);Study on the tissue culture of Capsicum annuum L.[J];Journal of Northeast Agricultural University;2008-11
5 SUN Zhi-qiang,GUO Ling-juan,HU Jian-bing,QI Wei-qiang(College of Foreatry and Horticulture,Henan Agricultural University,Zhengzhou,Henan 450002,China);Effects of Different Culture Factors on Callus Rate of Eggplant Anthers[J];Guizhou Agricultural Sciences;2009-04
6 LIU Juan1,2,TANG Hao-ru1,LIU Ling-mei1(1College of Forestry and Horticulture,Sichuan Agricultural University,Ya'an,Sichuan 625014 China;2Department of Economic Crops,Agricultural Bureau of Sichuan Province,Chengdu,Sichuan 610041 China);Effects of AgNO_3 on changes of endogenous polyamine contents in the process of adventitious bud formation in in vitro leaves of pear[J];Journal of Fruit Science;2008-06
7 JIA Xiu-ping1,WANG Han-ning1,ZHANG Jin-wen1,YUE Yun2(1.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;2.Gansu Agriculture Technology Extend Station,Lanzhou 730020,China);Influence of 2,4-D and NAA on embryonic callus induction from different explants in mature seeds of the maize parent K12[J];Journal of Gansu Agricultural University;2008-06
8 LUAN Xiao-min1,WANG Ming-jiu1*,HE Li-jun2,LI-Yao1,LI Zhi-qiang1(1.College of Ecology and Environmental Science,Inner Mongolia Agriculture University,Huhhot,Inner Mongolia Autonomous Region 010019,China;2.College of Agronomy,Inner Mongolia Agriculture University,Huhhot,Inner Mongolia Autonomous Region 010019,China);Screening of Embryogenic Callus from Hybrid F1 of Caucasian Clover×White Clover[J];Acta Agrestia Sinica;2009-05
9 YANG Li-huia,CHEN Yao-fengb,HAN Qiao-lingb,LI Chun-lianb(a.College of Animal Sciences and Technology,b.College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China);Establishment of the system of high frequency regeneration from hypocotyls of Alfalfa[J];Journal of Northwest A & F University(Natural Science Edition);2008-11
10 SU Yu-chun,HAN Wei-wei,CHEN Guang(College of Life Science,Jilin Agricultural University,Changchun 130118,China);Study on Establishment of Regeneration System of Alfalfa[J];Chinese Journal of Grassland;2008-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhou Zhongxin; Zhang Zongjiang; Liu Yianjun; Jiang Qianyun(Horticultural Department,Tianjin Agricultural College, Tianjin 300381 )Mi Jiengjiu(Biological College of Beijing Agricultural University, Beijing 100094);In vitro Plant Regeneration from Cotyledon Explants of Capsicum annuum[J];ACTA AGRICULTURAE BOREALL—SINICA;1994-02
2 Jiang Guoyong Xue Jing Zhang Yuna Lu Rongzhen (Genetics Laboratory, LAC) (Laiyang Seed Company);INDUCING EFFECT ON CALLI IN THE TRANSFORMATION SYSTEM IN POTATO[J];JOURNAL OF LAIYANG AGRICULTURAL COLLEGE;1998-03
3 Shuang Bao;Li Wenfu;Li Wenbin;Chen Yili;Tian Xingya;La Wenhe;Wang Fengyi;Zhu Xiangchun and Qin Xin(Northeast Agricultural University,Harbin 150030);THE CONSTRUCTION OF OPTIMIZED REGENERATION SYSTEM IN POTATO(SOLANUM TUBEROSUM L.)[J];CHINESE POTATO JOURNAL;1995-03
4 Zhou Jun, Xiao Ying, Cheng Bingquan (Central Laboratory of Xinjiang Academy of Agricultural Sciences, Urumqi 830000) Peng Xiangru and Liu Wenpu (Vegetable Institute of Urumqi, Xinjiang 830011);Shoot Tip Culture for Elimination Virus in Chilli[J];ACTA AGRICULTURAE BOREALI-OCCIDENTALIS SINICA;1996-03
5 He Xiaoming;Wang Ming;Wang Zhezhi(Department of Horticulture,Northwestern Agricultural University,Yangling,Shaanxi,712100)(Department of Biology,Shaanxi Normal University,Xi'an 710062);In Vitro Plant Regeneration from Leaf Explants of Pepper(Capsicum annuum L.)[J];ACTA UNIVERSITATIS AGRICULTURAE BOREALI-OCCIDENTALIS;1996-01
6 Gubaruowa Jiang pin;On the Organization Nurture of Chillies[J];Journal of Xingjiang Normal University(Natural Sciences Edition);1990-02
8 Dong Chunzhi, Jiang Chunxiao, Feng Lanxiang, and Guo Jiazhen ( Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081 );TRANSGENIC PEPPER PLANTS ( CAPSICUM ANNUUM L. ) CONTAINING CMV SAT-RNA cDNA[J];Acta Horticulturae Sinica;1992-02
9 Cao Dongsun and Jia Shirong (Biotechnology Research Center , Chinese Academy of Agricultural Sciences, Beijing 100081);In Vitro Plant Regeneration of Sweet Pepper[J];Acta Horticulturae Sinica;1993-02
10 Yu Xiaolin 1,Li Naijian 2,Huang Ziran 1,and Li Ying 2 (1Department of Sericulture South China Agricultural University,GuangZhou 510642; 2Vegetable Research Institute Guangdong Academy of Agricultural Sciences,GuangZhou 510640);In Vitro Shoot Differentiation from Cotyledon Explants of Pepper(Capsicum annuum L.)[J];ACTA HORTICULTURAE SINICA;2000-01
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