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《Gansu Agricultural Science and Technology》 2015-01
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Study on the Effect of Medium on Induction and Differentiation of Callus of Angelica sinensis

WANG Hong-xia;WANG Guo-xiang;CAI Zi-ping;Institute of Chinese Herbal Medicines,Gansu Academy of Agricultural Sciences;Gansu Engineering Laboratory for Genetic Improvement and Quality Control of Chinese Herbal Medicine;College of Agronomy,Gansu Agricultuarl Univercity;  
The seeds of Angelica sinensis are used to study the influence of different hormone combinations and media on callus induction and plant regeneration. The result shows that the optimal madium for callus induction is H+0.5 mg/L 2,4-D,and on it,the callus forming rate could reach to 97.92%;the optimal medium for induction of adventitious buds is 1/2 MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.5 mg/L ZT,and the highest induction rate is 90.3%;1/2 MS+0.5 mg/L IAA is the best for rooting,and the rooting rate could reach to 35.5%.
【Fund】: 甘肃省农业科技创新专项(2011GAAS06-8;2012GAAS05-1;2013GAAS03-2;2013GAAS03-2);; 甘肃省农业生物技术研究与应用开发项目(GNSW-2011-06)
【CateGory Index】: S567.239
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