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Effects of Anthopleurin-Qon the Intracellular Free Ca~(2+) Concentrationin Cultured Rat Cortical Astrocytes

LIUJue1,WANG Fang1,LONG Li-hong1,LIN Fan-kai1,YAO Wei-xing1,WANG Wei2,CHENJian-guo1(1.Depart ment of Pharmacology,Tongji Medical College,Huazhong University of Scienceand Technology,Wuhan 430030,China;2.Depart ment of Neurology,Tongji Hospital,TongjiMedical College,Huazhong University of Science and Technology,Wuhan 430030,China)  
Objective : Toinvestigate the effect of anthopleurin-Q(AP-Q) on the intracellular Ca2 +concentra-tion([Ca2 +]i) in cultured cortical astrocytes of rats.Methods :The [Ca2 +]i was monitored by calciumi maging withCa2 +sensitive fluorescent probe fura-2.Results : Aconcentration of 300 nmol/L AP-Qincreased the [Ca2 +]i in as-trocytes by(136.98 %±35.63 %)(n=28),when compared with the baseline level.Furthermore,the elevation of[Ca2 +]i was prevented by extracellular calcium free solution or when the extracellular Na+was replaced byNMDG+,and was decreased by Ni+,a non-specific antagonist of Na+/Ca2 +exchanger.Conclusion: AP-Qinducedthe intracellular [Ca2 +]i elevationin cultured rat cortical astrocytes via activating the reverse mode of Na+/Ca2 +ex-changer.AP-Q may be a useful tool to develop experi mental model of seizures.
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