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《Neural Injury and Functional Reconstruction》 2006-01
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Effects of Anthopleurin-Qon the Intracellular Free Ca~(2+) Concentrationin Cultured Rat Cortical Astrocytes

LIUJue1,WANG Fang1,LONG Li-hong1,LIN Fan-kai1,YAO Wei-xing1,WANG Wei2,CHENJian-guo1(1.Depart ment of Pharmacology,Tongji Medical College,Huazhong University of Scienceand Technology,Wuhan 430030,China;2.Depart ment of Neurology,Tongji Hospital,TongjiMedical College,Huazhong University of Science and Technology,Wuhan 430030,China)  
Objective : Toinvestigate the effect of anthopleurin-Q(AP-Q) on the intracellular Ca2 +concentra-tion([Ca2 +]i) in cultured cortical astrocytes of rats.Methods :The [Ca2 +]i was monitored by calciumi maging withCa2 +sensitive fluorescent probe fura-2.Results : Aconcentration of 300 nmol/L AP-Qincreased the [Ca2 +]i in as-trocytes by(136.98 %±35.63 %)(n=28),when compared with the baseline level.Furthermore,the elevation of[Ca2 +]i was prevented by extracellular calcium free solution or when the extracellular Na+was replaced byNMDG+,and was decreased by Ni+,a non-specific antagonist of Na+/Ca2 +exchanger.Conclusion: AP-Qinducedthe intracellular [Ca2 +]i elevationin cultured rat cortical astrocytes via activating the reverse mode of Na+/Ca2 +ex-changer.AP-Q may be a useful tool to develop experi mental model of seizures.
【CateGory Index】: R741
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Chinese Journal Full-text Database 4 Hits
1 OUYANG Ping 1 , DENG Chun-yu 2 , LIU Wen-hua 3 , WANG Lei 3 , LIANG Dong 3 , QIAN Wei-min 2 , XU An-long 31 Department of Cardiology, Nanfang Hospital, First Military Medical University, Guangzhou,510515, China; 2 Division of Cardiac Electrophysiology, Institutes of Cardiovascular Diseases of Guangdong Province, Guangzhou 510080, China; 3 The Open Laboratory for Marine Functional Genomics of State High-Tech Development, Department of Biochemistry, College of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China;Ionic mechanisms of a novel neurotoxin from the sea anemone Anthopleura sp.in rat ventricular myocytes[J];Journal of First Military Medical University;2004-06
2 ZHANG Lu, TANG Min-Feng, XIAO Ming, DING Jiong, HANG Qun-Ying (Department of Human Anatomy , Nanjing Medical University, Nanjing 210029, China);Distribution of astrocytes in hypothalamus of rat[J];Chinese Journal of Anatomy;2006-05
3 HU Zeng-Yao,YANG Sheng,ZHOU Wen-Xia,ZHANG Yong-Xiang(Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China);Advances in research on the effects of astrocyte on synaptic plasticity[J];Bulletin of the Academy of Military Medical Sciences;2007-03
4 ZHANG Lu, TANG Min-feng, LI Lei, HAN Qun-ying* (Department of Anatomy and Histology and Embryology, NJMU, Nanjing 210029, China);Reaction of astrocytes in paraventricular nucleus of thalamus and paraventricular nucleus of hypothalamus of rats after vagus nerve stimulation in rats[J];Acta Academiae Medicinae Nanjing;2005-07
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