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Effects of Pentylenetetrazol and Nuclear Factor kappa B Decoy Oligodeoxynucleotides on the Expression of Growth-associated Protein-43 in Neuron-like PC12 Cells

LI Wei-hua~*,ZHU Sui-qiang,TANG Rong-hua,GOU Yu-lan.~*Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China  
Objective: To investigate the effects of pentylenetetrazol(PTZ) and nuclear factor kappa B decoy oligodeoxynucleotides(NF-κB ODNs) on the expression of growth-associated protein-43(GAP-43)in neuron-like PC12 cell and to explore the epileptogenesis.Methods:The expression level of GAP-43 was studied by Western blot in neuron-like PC12 cells with and without PTZ treatment.NF-κB decoy ODNs were transfected into neuron-like PC12 cells with Lipofectamine-2000,and confocal laser scanning microscope(CLSM) was performed to investigate the activation of NF-κB.Results:(1) Western blot showed that the level of GAP-43 was significantly increased in neuron-like PC12 cells with PTZ treatment when compared to the control cells.(2) CLSM showed that the decoy ONDs decreased the activation of NF-κB in neuron-like PC12 cell,while Western blot showed that the decoy ONDs did not decrease the level of GAP-43.Conclusion: PTZ can enhance the expression of GAP-43 and active NF-κB in PC12 cells.NF-κB could not modulate GAP-43 expression.
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