Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Neural Injury and Functional Reconstruction》 2006-01
Add to Favorite Get Latest Update

Inducing Effect of FGF-2 in Combination with NT-3 on Differentiation of EGF-responsive Neural Stem Cells into Neurons

CONG Lin~*,ZHU Xiao-feng.~*Neuroscience Institute,Jiamusi University,Jiamusi 154002,China  
Objective: To compare the effects of FGF2 and NT-3 on the differentiation of EGF-responsive neural stem cells in cultures.Methods: NSCs were cultured in DMEM/F12 medium containing EGF.Nestin was detected by immunofluorescent labeling,and Brdu was used to show cell stages in division.EGF-responsive NSCs plated into 6 well culture-plates were divided into 4 groups: 20ng/ml FGF-2,40ng/ml NT-3,FGF-2+NT-3 and the control groups.10 days after NSCs were cultured,NSE expression could be detected using immunocytochemical and immunofluorescent staining.The number of NSE positive cells was both counted and the percentage of positive cells was obtained respectively.Results: Most of the cultured cells were nestin-positive and Brdu-positive.Both FGF-2 and NT-3 can induce EGF-responsive NSCs to differentiate into neurons in vitro.The effect of FGF-2 on differentiation is more optimal than that of NT-3.The number of differentiated neurons in NT-3、FGF-2、FGF-2+ NT-3 and the control groups were 10.52%、19.69%、34.10%、3.70% respectively.Combination of NT-3 and FGF-2 could increase differentiation by about 10 times.Conclusion: Combination of FGF-2 and NT-3 in inducing differentiation of NSCs into neurons in vitro is recommended.
【Fund】: 国家自然科学基金(30540062);; 黑龙江省杰出青年基金(JC-02-05)
【CateGory Index】: R329.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 SHI Yong-jiang,LIU Hong-liang,YAO Zhong-xiang,et al.Department of Rehabilitation Medicine,Xinan Hospital,Third Military Medical University,Chongqing 400038,China;Effect of Ginsenoside Rg1,Rb1 on Proliferation of SVZa-NSCs and Expression of STAT3[J];Chinese Journal of Rehabilitation;2007-02
2 PENG li,PENG Rui,LV Jun,et al.Department of Acupuncture,Taihe Hospital,Yunyang Medical College,Shiyan 442000,China;Effect of Electric Acupuncture in Combination with Exercise Training on Nerve Functional Recovery and Expression of NGF after Cerebral Ischemia-Reperfusion in Rats[J];Chinese Journal of Rehabilitation;2007-06
Chinese Journal Full-text Database 1 Hits
1 CHEN Ling, CHEN Jing-gang, TANG Zhou-ping, et al. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Comparison of the Proportion of the Neurons in the Differentiated Cells from the Neural Stem Cells under Different Conditions[J];Chinese Journal of Rehabilitation;2005-02
Chinese Journal Full-text Database 4 Hits
1 LIU Zhi-ming, LONG Xing. (Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Wuhan University, Wuhan 430079, China);Research progress of embryonic stem cells differentiation into neural stem cells[J];International Journal of Stomatology;2009-06
2 ZHANG Hong-yan,GUO Yun-qiu~*,MA Hong-mei(Department of Histology and Embryology,Harbin Medical University,Heliongjiang Harbin 150081 China);Co-culture of Granular Convoluted Tubule(GCT) Cells of Rat Submandibular Gland and Neural Stem Cells(NSCs) of Fetal Rat Spinal Cord[J];Progress of Anatomical Sciences;2006-04
3 WU Qiang, LI Lu-si. The Department of Neurology, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China;Advance in Study of Neural Stem Cells Used for Treatment of Alzheimer's Disease (review)[J];Chinese Journal of Rehabilitation Theory and Practice;2006-10
4 ZHANG Lin,LIU Guang-yi.College of Marine Life Sciences,Ocean University of China,Qingdao 266002,China;Marrow Stem Cells Transplantation and Its Correlation with Expression of Growth Associated Protein-43 in Rats with Cerebral Ischemia Injury[J];Chinese Journal of Rehabilitation;2011-03
Chinese Journal Full-text Database 7 Hits
1 Fang Gui-lun,et al;The Proliferation Effects of Ginsenosides on Human Bone[J];ZHEJIANG CLINICAL MEDICAL JOURNAL;2000-02
2 WANG Zhaohui, XIAN Shaoxiang, YANG Zhongqi, LI Nanyi, ZHAO Licheng (Doctoral Graduate of Grade 2003, Guangzhou University of TCM, Guangzhou 510405, China; The First Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510405, China);Inductive Effect of Ginsenosides on Differentiation of Rat Marrow Mesenchymal Stem Cells into Myocardial Cells in Vitro[J];Journal of Guangzhou University of Traditional Chinese Medicine;2006-02
3 ZHANG Hui-lai *, WANG Hua-qing, YAO Zhi, et al. *Tianjin Cancer Hospital and Institute,Tianjin Medical University 300060,China;;Study on effect of ginsenoside Rg3 on immunological recovery after peripheral blood stem cell transplantation in animal experiments[J];Chinese Journal of Radiation Mediation and Protection;2005-05
4 LIAO Weijing *, LIU Shuhong, FAN Ming, YANG Wantong, YANG Yunhuang, LIU Maili. *Department of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China;Modification of the preparation of models of focal ischemic brain injury with suture occlusion in rats[J];Chinese Journal of Physical Medicine and Retabulitation;2002-06
5 PENG Li,LI Lai-you, PENG Rui, et al. Taihe Hospital of Shiyan, S hiyan 442000,China;Electroacupuncture in Combination with Promote Treatment Technique for the Recovery of Ambulation in the Patients with Acute Apoplexy[J];Chinese Journal of Rehabilitation;2004-01
6 JIN Jinmei, Tao Helen, GAO Ruilan, et al The Affiliated Hospital of Zhejiang College of TCM, Hangzhou (310006);Effect of Ginsenosides on Proliferation and Differentiation of Human CD_(34)~+ Hematopoietic Stem/Progenitor Cells[J];CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE;2000-09
7 WANG Ning-yuan, LU Chuan-jiang, CHEN Xue-hai, et al Department of Cardiology Regeneration Medicine, Gifu University School of Medicine, Gifu, Japan;Study on Effect of Ginsenoside Rg1 in Promoting Myocardiac Vascular Endothelial Cell Regeneration through Induction on Bone Marrow Stem Cell's Migration and Differentiation in Rabbits of Myocardial Infarction[J];Chinese Journal of Integrated Traditional and Western Medicine;2005-10
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 JIA Ji-ming WANG Zong-quan WU Li-jun WU Yi-ling (1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shengyang 110016,China; 2.Hebei Province Traditional Chinese Medicine and Western Medicine Institute of Pharmaceutical,Shijiazhuang 050035,China);Advance of pharmacological study on ginsenoside Rb_1[J];China Journal of Chinese Materia Medica;2008-12
2 LOU Jiang-yan,NI Hong,TAO Lu-yang,et al.Department of Pediatrics,Affiliated Children's Hospital of Soochow University,Suzhou 215003,China;Long-term Effects of Physical Exercise on Learning Ability and ZnT1 Expression in Hippocampus of Rats with Neonatal Seizures[J];Chinese Journal of Rehabilitation;2009-04
3 Ma Ping1,Tang Qiang2:1. Daqing Division,Harbin Medical University,Daqing 163319,China;2. Heilongjiang University of Chinese Medicine,Harbin 150040,China;Effect of Acupuncture Combined with Rehabilitation on Learning and Memory in Vascular Dementia (VD) Rats and the Expression of CHAT in Hippocampus[J];Information on Traditional Chinese Medicine;2009-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved