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Inducing Effect of FGF-2 in Combination with NT-3 on Differentiation of EGF-responsive Neural Stem Cells into Neurons

CONG Lin~*,ZHU Xiao-feng.~*Neuroscience Institute,Jiamusi University,Jiamusi 154002,China  
Objective: To compare the effects of FGF2 and NT-3 on the differentiation of EGF-responsive neural stem cells in cultures.Methods: NSCs were cultured in DMEM/F12 medium containing EGF.Nestin was detected by immunofluorescent labeling,and Brdu was used to show cell stages in division.EGF-responsive NSCs plated into 6 well culture-plates were divided into 4 groups: 20ng/ml FGF-2,40ng/ml NT-3,FGF-2+NT-3 and the control groups.10 days after NSCs were cultured,NSE expression could be detected using immunocytochemical and immunofluorescent staining.The number of NSE positive cells was both counted and the percentage of positive cells was obtained respectively.Results: Most of the cultured cells were nestin-positive and Brdu-positive.Both FGF-2 and NT-3 can induce EGF-responsive NSCs to differentiate into neurons in vitro.The effect of FGF-2 on differentiation is more optimal than that of NT-3.The number of differentiated neurons in NT-3、FGF-2、FGF-2+ NT-3 and the control groups were 10.52%、19.69%、34.10%、3.70% respectively.Combination of NT-3 and FGF-2 could increase differentiation by about 10 times.Conclusion: Combination of FGF-2 and NT-3 in inducing differentiation of NSCs into neurons in vitro is recommended.
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