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Resistance of the Transgenic CHO Cell to Toxicity

WANG Ming-wei~*,GU Ping,CUI Dong-sheng,WANG Yan-yong,LIU li.~*Department of Neurology,or Brain Ageing and Cognitive Neuroscience Laboratory of Hebei Province,The First Hospital of Hebei Medical University,Shijiazhuang 050031,China  
Objective:The toxicity to transgenic CHO of vesicular monoamine transporter 2(VMAT_2) and to wild type CHO generated by MPP~+ and Rotenone was assessed.Methods: MTT assay was used to detect cell survival rate of both WT-CHO and VMAT_2-CHO following exposure to MPP~+ and Rotenone.Meanwhile the change of cell morphology was observed.Fluorescence intensity of intracellular Ca~(2+) concentration in the two groups of cells was measured under the Confocal laser microscopy.Results:Transgenic VMAT_2-CHO cells were resistent to the toxicity of MPP~+(the concentration range: 0.2-2.0 mmol/L) and Rotenone(the concentration range: 0.05-(1.0μmol/L)).Conclusion: Transgenic CHO cells with VMAT2 can resist the toxicity caused by Rotenone and MPP~+.
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