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《Neural Injury and Functional Reconstruction》 2006-02
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Changes in Gene Expressions Induced by Nerve Regeneration Factor in Cultured Hippocampal Neurons Detected with DNA Microarray

CHEN Yi-ren~▲, TANG Xin, ZHANG Qi, DING Fei, GU Xiao-song . ~▲Key Laboratory of Neuroregeneration of Jiangsu Province, Nantong University,Nantong 226001, China  
Objective: To study mechanisms of growth-promoting effects of Chinese herb "Nerve regeneration factor" (NRF) on hippocampal neurons. Methods: Rat embryonic (17d) hippocampal neurons were cultured in the presence or absence of NRF (1ug/ml). Neurite length was measured and the total RNAs were extracted and purified from control and NRF-treated neurons. The fluorescent cDNA probes were prepared through reverse transcription and hybridized with DNA microarry chips. The chips were then scanned and analyzed. Results: The averaged neuritic length of neurons treated with NRF in the NRF-treated neurons was significantly longer than that in the control (P0.01). Out of total 15866 genes examined, 249 were significantly up regulated and 316 were down regulated in NRF treated neurons. These differentially expressed genes induced by NRF include G-protein coupled receptors, signal transduction molecules, cell growth and metabolism regulating factors, cellular structure molecules, enzymes, immuno-globulins, and apoptosis signaling molecules. Conclusion: NRF promotes neurite growth and regulates gene expression of a broad range of important signaling molecules. DNA microarray analysis provides a sensitive approach for preliminary searching of the targets of NRF effects.
【Fund】: 973国家自然科学基金项目(2003CB515306)
【CateGory Index】: R285.5
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