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《Neural Injury and Functional Reconstruction》 2006-02
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Construction of the Lentiviral Expression Vector Carrying Mash1 Gene

ZHANG Rui~▲, XU Yu-ming, ZHAO Guo-qiang, GUO Jian-xin, SONG Bo, ZHANG Shi-jie,HAN Zhi-qiang.  
Objective:To construct the lentiviral expression vector of murine Mash1 for further study on neural development and neural differentiation. Methods:The entire Mash1 cDNA was amplified by RT-PCR from a mouse embryo and then was ligated with pGEM-T vector. The ligation product was transformed into the JM109 competent cells. The positive recombinant clones were selected and identified by α-complementation, PCR, restriction endonuclease digestion and DNA sequencing. The cloning vector and the Lentivirus were cut by BamHⅠand XholⅠ. Then they were ligated and transformed. The enzyme analysis was used to confirm the recombinant vector. Results:The results of PCR, enzyme analysis and DNA sequencing analysis have confirmed that the right Mash1 gene was cloned. Conclusion:The cDNA clone of murine Mash1 and its lentiviral expression vector Lentivirus-Mash1 are successfully constructed.
【Fund】: 国家自然科学基金资助项目(30570624);; 河南省教委杰出人才资助项目;; 郑州市重大科技攻关项目(03BA02BMYB05)
【CateGory Index】: R346
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