Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Apoptosis of PC12 Cells Induced by H_2O_2 is Correlated with Down-regulated Expression of 14-3-3 Protein

CHEN Xiao-wu,SU Qing-jie,CHEN Zhi-bin,SUN Sheng-gang,QIAO Xian.Department of Neurology,Affiliated hospital of Hainan Medical College,Haikou 570102,China  
Objective:To explore optimal concentration and time-course of H_2O_2 treatment of PC12 cells for setting up an oxidative stress cell model of PD,and to study relationship between apoptosis of PC12 cells induced by H_2O_2 and expression level of 14-3-3 protein.Methods:MTT assay,fluorescent spectrophotometer,flow cytometric analysis,and western blotting were employed to measure cell viability,caspase activity,apoptotic ratio,and expression level of 14-3-3 protein respectively.Results:Treating PC12 cells with 100 μmol/L for 24 hours yielded the highest caspase activity and apoptotic ratio.A significantly negative correlation was found between expression level of 14-3-3 protein and apoptotic ratio of PC12 cells induced by H_2O_2.Conclusion:Incubation with 100 μmol/L H_2O_2 for 24 hours is the optimal condition for setting up the oxidative stress cell model of PD.The apoptosis of PC12 cells induced by H_2O_2 is correlated with the down-regulated expression of 14-3-3 protein.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved