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Statins Reduce Intracellular Amyloid β Aggregation

LI Xiao-qing,WANG Li,WANG Li-feng.Department of Neurology,Beijing An Zhen Hospital of the Capital University of Medical Sciences,Beijing 100029,China  
Objective:To investigate the effect of Statins on intracellular Amyloid β(Aβ) aggregation.Methods: SH-SY5Y cells were divided into groups control,transfection,Atorvastatin and Simvastatin.The control group received no treatment,and the other 3 groups were transfected by plasmid pcDNA3.0-YFP-C99 to construct Alzheimer's disease(AD) cell models.The Atorvastatin group and Simvastatin group were added Atorvastatin and Simvastatin at different concentration(5、10、20、50、100 μmol/L) respectively.The Aβ changes in all the groups were observed with fluorescence microscope and quantitatively analyzed with ELISA assay and Western blot before and 12,24,48 and 72 h after culture.Results: In the pathogenic process of AD,A initially accumulated within the cells.By observation with fluorescence microscopy,intracellular Aβ was significantly reduced by statin treatment in a dose-and time-dependent manner.The Aβ reduction was statistically significant among the groups by 20 μmol/L statin treatment for 24 h(P0.05).Conclusion: Aβ aggregation occurs initially in the AD cell model.Different types of statins could decrease Aβ accumulation in a dose-and time-dependent manner.
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