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《Neural Injury and Functional Reconstruction》 2014-01
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Effect of TREK-1 Activation on Cell Apoptosis and the Expression of Apoptosis-associated Protein in Rats Following Focal Cerebral Ischemia

LIU Yang;SUN Qian;WANG Wei;XIE Min-jie;Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology;  
Objective:To investigate the effect of TREK-1 channel activity on cell apoptosis and the protein expression of Bcl-2 and Bax after focal cerebral ischemia in rats. Methods: Forty-five rats were randomly assigned into sham-operated(n=10), vehicle-treatment(n=10), and intervention groups(n=25). Photochemical ischemia was performed and sham-operated animals were given saline instead of Rose Bengal. Alpha-lineonic acid was administrated i.c.v(100 μmol/L, 250 μmol/L, 500 μmol/L, or 1 mmol/L). Vehicle treated animals received the same volumes of 0.9% NaCl. The cellular distribution of TREK-1 in rat brain was detected by immunohistochemical staining. Double staining of TUNEL and DAPI was performed to determine the percentage of cell death and the protein expression of B-cell lymphoma-2(Bcl-2), Bcl-2-associated X protein(Bax) and Phosphorylated Extracellular Signal-Regulated Kinase(p-erk) was detected by western blot. Results: TREK-1 immunoreactivety was expressed in the GFAP positive astrocytes and NeuN positive neurons in the cerebral cortex. Compared with sham-operated groups, the percentage of TUNEL positve cells and the phosphorylation of erk increased significantly, the Bcl-2 to Bax ratio decreased dramatically(P0.05). However, after treatment with LIN, the percentage of TUNEL positive cells and the phosphorylation of erk was reduced significantly compared to that of vehicle group, and the Bcl-2/ Bax ratio was increased(P0.05). Conclusion: TREK-1 was expressed in astrocytes and neurons. TREK-1 activation with LIN remarkably inhibited the cell apoptosis and the phosphorylation of erk, and increased the ratio of protein expression of Bcl-2 and Bax following focal cerebral ischemia.
【Fund】: 国家自然科学基金(No.30971007);国家自然科学基金重点项目(No.81030021)
【CateGory Index】: R743.31
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Chinese Journal Full-text Database 4 Hits
1 WU Xiao▲,XIE Min-jie,TANG Rong-hua.▲Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Expression of TREK-1 on Cultured Neuron and Astrocytes under Normal and Hypoxia Condition[J];Neural Injury and Functional Reconstruction;2012-03
2 Xiao WU1, Ronghua TANG 1, Yang LIU 1, Jingjiao SONG 2, Zhiyuan YU 1, Wei WANG 1, Minjie XIE 1 1Department of Neurology, 2Division of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Small RNA Interference-mediated Gene Silencing of TREK-1 Potassium Channel in Cultured Astrocytes[J];华中科技大学学报(医学英德文版);2012-06
3 WU Chuan-xia1,FANG Hao1,FENG Ya-ning1,BAI Fei-hu2 1.Postgraduate College,Ningxia Medical University,Yinchuan 750004,P.R.China 2.Department of Gastroenterology,Ningxia People's Hospital,Yinchuan 750002,P.R.China;Effects of ApoG2on proliferation and apoptosis of gastric cancer cells SGC7901[J];Chinese Journal of Cancer Prevention and Treatment;2013-09
4 LE Ke-jia,SHI Yi-ming,XIE Yi-fan,LU Yang (Department of Pharmacy,Basic Medical College,Shanghai Jiaotong University,Shanghai 200025,China);Quality control and stability determination with HPLC-ED of N-stearoyltyrosine[J];Journal of Shanghai Jiaotong University(Medical Science);2013-08
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