Expression and Subcellular Localization of KCNQ1 in Stria Vascularis of Mice Cochlea by Laser Scanning Confocal Microscopy and Immunohistochemistry Staining
WU Zhen-gong;CHU Han-qi;XIONG Jun;CHEN Jin-chuan;LIU Yun;CHEN Jin;ZHOU Liang-qiang;XIONG Hao;Department of ENT,Anxi Hospital;
Objective:To investigate the expression and distribution of KCNQ1 in stria vascularis of murine cochlea with laser scanning confocal microscopy(LSCM) and immunohistochemistry staining technique. Methods: With LSCM and immunohistochemistry staining, the location of KCNQ1 in cochlea of the CBA/CaJ and KCNQ1-/- mice were observed. Results: Immunohistochemical staining showed that the expression of KCNQ1 protein was found at the apical membrane wall of marginal cells in stria vascularis of CBA/CaJ mice. There was no KCNQ1 protein expression in marginal cells of KCNQ1-/- mice. The fluorescence of KCNQl protein was mainly expressed in the cell membrane of marginal cells of CBA/CaJ mice with LSCM. Conclusion: The expression and distribution of KCNQ1 protein in marginal cells of stria vascularis could be observed with LSCM.